5-Aza-CdR对HT22小鼠海马神经元细胞中NR2B磷酸化的影响  

Effects of 5-Aza-CdR on p-Y1472 NR2B in the HT22 cells

作　　者：姜树原 王强[2] 张晓璐 刘友[2] 邵国[1,3] JIANG Shuyuan;Wang Qiang;Zhang Xiaolu;Liu You;Shao Guo(Inner Mongolia Key Laboratory of Hypoxic Translational Medicine of Baotou Medical College,Baotou 014040;Department of Basic Medicine and Forensic Medicine of Baotou Medical College;Beijing Key Laboratory of Hypoxic Conditioning Translational Medicine of Capital Medical University)

机构地区：[1]内蒙古科技大学包头医学院内蒙古自治区低氧转化医学重点实验室,内蒙古包头014040 [2]内蒙古科技大学包头医学院基础医学与法医学院 [3]首都医科大学低氧医学研究所

出　　处：《包头医学院学报》2020年第5期40-42,共3页Journal of Baotou Medical College

基　　金：国家自然科学基金项目(81660307);内蒙古自然科学基金项目(2020MS08006,2016MS(LH)0307,2019MS08052,2020MS08006);内蒙古自治区高等学校科研项目(NJZY17250,NJZY17251);包头医学院科学研究基金项目(BYJJ-YF 201615,BYJJ-YF 201719,2018BYJJ-HL-06)。

摘　　要：目的:研究5-氮杂-2′-脱氧胞苷对HT22小鼠海马神经元细胞中NR2B 1472位点的酪氨酸磷酸化(p-Y1472 NR2B)的影响。方法:使用小鼠海马神经元细胞系HT22,对照组细胞用0.1%BSA处理,处理组细胞用10μmol/L 5-Aza-CdR处理。采用real time RT-PCR检测NR2B mRNA的表达;Western Blot检测NR2B和磷酸化NR2B-Y1472(p-Y1472 NR2B)的表达水平;MTS法检测细胞活力;流式细胞分析技术检测细胞周期。结果:HT22细胞在10μmol/L 5-Aza-CdR作用下,NR2B-Y1472的磷酸化水平升高,细胞活力下降,细胞周期受到阻滞。结论:5-Aza-CdR对小鼠行为记忆能力的影响可能与NR2B-Y1472磷酸化相关。Objective:To study the effects of 5-Aza-2'-deoxycytidine on the tyrosine phosphorylation of NR2B 1472 site(p-Y1472 NR2B)in the HT22 cells.Methods:The mice hippocampal neuron cell line HT22 was used in vitro experiment,cells in the control group were treated with 0.1%BSA,and the treatment group were treated with 10μmol/L 5-Aza-CdR.The mRNA level of NR2B were measured by real time RT-PCR.NR2B and p-Y1472 NR2B were measured by Western Blot.Cell activity was detected by MTS method.Cell cycle was detected by flow cytometry.Results:In vitro experiments showed that 10μmol/L 5-Aza-CdR can increased the phosphorylation level of NR2B-Y1472,decreased the cell viability and arrested cell cycle.Conclusions:The effects of 5-Aza-CdR on behavioral memory ability may be related to the phosphorylation NR2B-Y1472.

关 键 词：5-AZA-CDR NR2B 磷酸化 

分 类 号：R338[医药卫生—人体生理学]