墨吉明对虾(Fenneropenaeus merguiensis)RACK基因的克隆与表达分析  

Cloning and Expression Analysis of RACK Gene of Fenneropenaeus merguiensis

作　　者：雷易果 周婷婷[1] 梁庆展 陈兆明[1] Lei Yiguo;Zhou Tingting;Liang Qinzhan;Chen Zhaoming(College of Fisheries,Guangdong Ocean University,Zhanjiang,524088)

机构地区：[1]广东海洋大学水产学院,湛江524088

出　　处：《基因组学与应用生物学》2020年第6期2462-2469,共8页Genomics and Applied Biology

基　　金：国家自然科学基金委(#31572606)项目基金资助。

摘　　要：RACK基因作为鸟嘌呤核苷酸结合蛋白β亚基中的部分基因,主要编码一种神经肽信号分子,在膜结合受体和细胞内效应器之间起信号传递作用。为了研究RACK基因在墨吉明对虾各个组织和发育周期中的表达,本实验通过基因克隆技术获得墨吉明对虾RACK基因ORF序列,共957 bp,编码318个氨基酸,并通过荧光定量PCR技术检测RACK基因在墨吉明对虾不同组织的表达量变化。数据分析表明,RACK基因在墨吉明对虾各个部位都有表达,其中在眼柄表达量最高,在胸节神经和血液中表达量次之,在肌肉表达量最低(p>0.05)。RACK基因在墨吉明对虾整个生长周期中都有表达,且存在显著差异,总体来看糠虾时期的RACK基因表达量明显高于无节幼体、溞状幼体和仔虾时期(p>0.05)。本研究为更加深入研究RACK基因的功能与表达分析提供理论基础。As part of the guanine nucleotide binding protein,RACK gene is mainly used as a neuropeptide signal molecule,which plays a signal transmission role between the membrane binding receptor and the intracellular effectors.In order to study the expression of RACK gene in different tissues and development cycles of Fenneropenaeus merguiensis,this experiment obtained the RACK gene ORF and its length was 957 bp,which encoded 318 amino acids and sequence of medin prawns through gene cloning technology,and detected the change of RACK gene expression in different tissues of medin prawns through fluorescence quantitative PCR.Data analysis showed that the RACK gene was expressed in all parts of the prawns,with the highest expression in the eye stalk,the second highest expression in the thoracic ganglion and blood,and the lowest expression in the muscle(p>0.05).RACK gene was expressed throughout the growth cycle of Fenneropenaeus merguiensis,and there were significant differences.In general,the expression of RACK gene in mysis was significantly higher than that in nodal larva,daphnia larva and larva prawns(p>0.05).This study could lay a simple theoretical foundation for further study on the function and expression analysis of RACK gene.

关 键 词：墨吉明对虾 RACK基因 克隆 表达 

分 类 号：S917.4[农业科学—水产科学]