白花蛇舌草对人胃癌MKN-45细胞周期的影响  

Effect of Hedyotis diffusa on Proliferation of Human Gastric Cancer MKN-45 Cells

作　　者：代飞 邵淑丽[1,2] 焦凯贺 冯元 张伟伟[1,2] 张珍珠[1,2] 崔婷婷 李铁[1] Dai Fei;Shao Shuli;Jiao Kaihe;Feng Yuan;Zhang Weiwei;Zhang Zhenzhu;Cui Tingting;Li Tie(College of Life Science and Agriculture and Forestry,Qiqihar University,Qiqihacr,161006;Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,Qiqihaer,161006)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,齐齐哈尔161006 [2]抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,齐齐哈尔161006

出　　处：《基因组学与应用生物学》2020年第6期2866-2872,共7页Genomics and Applied Biology

基　　金：黑龙江省自然科学基金(批准号:C201241);黑龙江省教育厅基本业务专项重点项目(批准号:135109104);黑龙江省教育厅基本业务专项(粮头食尾,批准号:LTSW201737)共同资助;黑龙江省省属高等学校基本科研业务费科研项目(植物性食品加工技术特色学科专项,批准号:YSTSXK201809)。

摘　　要：为了寻找能抑制肿瘤细胞增殖的药物,本研究探讨了不同浓度白花蛇舌草(Hedyotis diffusa)对人胃癌MKN-45细胞的影响,使用不同浓度的白花蛇舌草处理人胃癌MKN-45细胞24 h、48 h、72 h,共聚焦显微镜下观察细胞形态,流式细胞术检测细胞周期,qRT-PCR和Western blotting检测CCNB1和CCND1的表达水平。结果表明,在一定浓度范围内,白花蛇舌草可抑制人胃癌MKN-45细胞增殖且呈剂量时间依赖性;白花蛇舌草处理人胃癌MKN-45细胞24 h、48 h、72 h后的IC50值分别为111.759μg/mL、26.878μg/mL、16.179μg/mL;随着白花蛇舌草的浓度增加,显微镜视野下细胞数量减少;G1期细胞减少,S期细胞增多;CCNB1和CCND1基因表达水平显著降低。白花蛇舌草能能抑制人胃癌MKN-45细胞增殖,阻滞细胞于S期,降低CCNB1和CCND1基因的表达。In order to find a drug that can inhibit the proliferation of human gastric cancer cells,the effects of different concentrations of Hedyotis diffusa on human gastric cancer MKN-45 cells were studied in this paper.Human gastric cancer MKN-45 cells were treated with different concentrations of Hedyotis diffusa for 24 h,48 h and 72 h.Cell morphology was observed under confocal microscope,flow cytometry was used to detect cell cycle,q RT-PCR and Western blotting was used to detect the expression of CCNB1 and CCND1 genes.The results showed that the proliferation of human gastric cancer MKN-45 cells could be inhibited in a dose-dependent manner in a certain concentration range.The IC50 values of gastric cancer MKN-45 cells were 111.759μg/mL,26.878μg/mL and 16.179μg/mL for 24 h,48 h,72 h that treated with Hedyotis diffusa,the number of cells decreased under microscope;G1 phase cell reduced,S phase cells increased;The expression level of CCNB1 and CCND1 genes decreased significantly.Conclusion:Hedyotis diffusa can inhibit the proliferation of human gastric cancer MKN-45 cells,block the cells in S phase,and decrease the expression of CCNB1 and CCND1 genes.

关 键 词：白花蛇舌草 MKN-45细胞 流式细胞术 CCNB1 CCND1 

分 类 号：R285.5[医药卫生—中药学]