腺苷脱氨酶基因多态性与反复自然流产的相关性  

Relationship between adenosine deaminase gene polymorphism and recurrent spontaneous abortion

作　　者：孙丽芳[1] 郑秀丽[1] 陈光雪 王新宓 刘静芳[1] SUN Li-fang;ZHENG Xiu-li;CHEN Guang-xue;WANG Xin-mi;LIU Jing-fang(Department of Obstetrics and Gynecology,Beijing Jishuitan Hospital,Beijing 100035,China)

机构地区：[1]北京积水潭医院妇产科,北京100035

出　　处：《中国临床研究》2020年第8期1080-1083,共4页Chinese Journal of Clinical Research

基　　金：吴阶平医学基金会-遗传与优生专项基金(320.6750.17022)。

摘　　要：目的探讨腺苷脱氨酶(ADA)基因多态性与反复自然流产(RSA)的相关性。方法随机选取2016年1月至2018年1月北京积水潭医院医院收治的孕妇300例,其中RSA患者120例作为试验组,无RSA患者180例作为对照组,收集患者临床资料,采集患者静脉血,以聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测ADA G22A基因多态性。结果试验组和对照组ADA基因型和等位基因的频率无统计学差异(P>0.05)。并存糖尿病、高血压、多囊卵巢综合征、子宫畸形、抗磷脂抗体阳性、子宫内膜异位症的RSA孕妇120例中ADA基因型频率分布无统计学差异(P>0.05)。在年龄≤35岁者中,两组ADA基因型和等位基因的频率分布无统计学差异(P>0.05)。在年龄>35岁者中,两组ADA基因型频率分布有统计学差异(P=0.005),GG基因型相对于GA基因型RSA的危险度(OR=0.113,95%CI=0.022~0.585);两组等位基因频率分布有统计学差异(P=0.001),G等位基因相对于A等位基因的RSA危险度(OR=9.774,95%CI=2.051~46.574)。结论年龄超过35岁的高龄孕妇中,ADA基因多态性与与RSA的发生密切相关,对高龄孕妇是否会发生流产具有一定的预判价值。Objective To explore the relationship between adenosine deaminase(ADA)gene polymorphism and recurrent spontaneous abortion(RSA).Methods A total of 300 patients admitted to Beijing Jishuitan Hospital from January 2016 to January 2018 were randomly selected,including 120 RSA patients as experimental group and 180 non-RSA patients as control group.After collecting the patients's venous blood,ADA G22A gene polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Results There were no significant differences in genotype and allele frequencies of ADA between two groups(P>0.05);and also no significant differences found in frequency distribution of ADA genotype of pregnant women complicated with diabetes mellitus,hypertension,polycystic ovary syndrome,uterine malformation,positive anti-phospholipid antibody and endometriosis in experimental group(P>0.05).There was no significant difference in genotype and allele frequency distribution of ADA in the patients aged less than 35 years between two groups(P>0.05),but statistical differences were obsewed in them for the patients aged more than 35 years between two groups(P=0.005).Compared with GA genotype,GG genotype significantly increased the risk for RSA(OR=0.113,95%CI=0.022-0.585).There was a statistical difference in allele frequency distribution between two groups(P=0.001).Compared with A allele,G allele showed a statistically increased risk for RSA(OR=9.774,95%CI=2.051-46.574).Conclusions The polymorphism of ADA gene is closely related to the occurrence of RSA in the pregnant women over 35 years old,which has a certain predictive value for the possibility of abortion in older pregnant women.

关 键 词：腺苷脱氨酶 基因多态性 反复自然流产 年龄 

分 类 号：R714.21[医药卫生—妇产科学]