基于染色体片段置换系对水稻粒形及千粒重QTL检测与稳定性分析  

QTL detection and stability analysis of rice grain shape and thousand-grain weight based on chromosome segment substitution lines

作　　者：王小雷 李炜星 曾博虹 孙晓棠[1] 欧阳林娟[1] 陈小荣[1] 贺浩华[1] 朱昌兰[1] WANG Xiao-Lei;LI Wei-Xing;ZENG Bo-Hong;SUN Xiao-Tang;OU-YANG Lin-Juan;CHEN Xiao-Rong;HE Hao-Hua;ZHU Chang-Lan(Key Laboratory of Crop Physiology,Ecology and Genetic Breeding(Jiangxi Agricultural University),Ministry of Education/Jiangxi Super Rice Engineering Technology Research Center,Nanchang 330045,Jiangxi,China;Jiangxi Super Rice Research and Development Center of Jiangxi Academy of Agricultural Sciences,Nanchang 330200,Jiangxi,China)

机构地区：[1]江西农业大学作物生理生态与遗传育种教育部重点实验室/江西省超级稻工程技术研究中心,江西南昌330045 [2]江西省农业科学院/江西省超级水稻研究发展中心,江西南昌330200

出　　处：《作物学报》2020年第10期1517-1525,共9页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31860373);国家转基因生物新品种培育重大专项(2016-ZX08001-002);江西省“5511”优势科技创新团队项目(2016-5BCB19005)资助。

摘　　要：粒形及千粒重是水稻产量的重要影响因素,通过挖掘这些性状的优异基因,对水稻超高产育种具有重要意义。本研究利用1套以籼稻恢复系昌恢121为背景亲本,粳稻越光为供体亲本构建的染色体片段代换系为材料,在3个环境下对水稻粒形及千粒重进行QTL检测及稳定性分析,共检测到59个QTL,分布于1号、2号、3号、4号、5号、6号、7号、10号、11号和12号染色体上,贡献率为0.77%~36.26%,其中发现10个QTL多效位点。值得关注的是qGW2-1、qGW2-2、qGW3-1、qGW3-2、qGL3和qGL12这6个QTL能在3个环境中重复检测到,其中qGW3-1为新鉴定的QTL位点。这些结果为进一步开展水稻粒形基因的精细定位、克隆和分子辅助育种奠定了一定的理论基础。Grain shape and 1000-grain weight are the important factors affecting rice yield.Discovering the excellent genes of these traits is of great significance for super high yield rice breeding.In this study,a set of chromosome segment substitute lines(CSSLs),derived from a cross between Koshihikari(a japonica cultivar,as donor patent)and Changhui 121(an indica restorer line,as a background patent),were used to quantitative trait locus(QTLs)detection and stability analysis in three environments.The results showed that a total of 59 QTLs were identified on chromosomes 1,2,3,4,5,6,7,10,11,and 12,respectively,whose contribution rate was 0.77%–36.26%.Among them,10 pleiotropic QTLs were found,and qGW2-1,qGW2-2,qGW3-1,qGW3-2,qGL3,and qGL12 could all be detected in three environments.Furthermore,qGW3-1 is a novel identified QTL locus.These results lay a foundation for further fine mapping,cloning and marker-assisted breeding of grain shape genes.

关 键 词：水稻 染色体片段置换系 粒形 千粒重 数量性状座位 

分 类 号：S511[农业科学—作物学]