多级微管结构骨支架载体促进成骨成血管作用及机制  

Role and mechanism of hierarchical microchanneled bone scaffolds in promoting osteogenesis and angiogenesis

作　　者：叶海民 丁凌华 孔维豪 黄祖泰 熊龙[1] Ye Haimin;Ding Linghua;Kong Weihao;Huang Zutai;Xiong Long(Department of Orthopedics,Jiangxi Provincial People’s Hospital,Nanchang 330000,Jiangxi Province,China;People’s Hospital Affiliated to Nanchang University,Nanchang 330000,Jiangxi Province,China;Department of Orthopedics,Gannan Medical University,Ganzhou 341000,Jiangxi Province,China)

机构地区：[1]江西省人民医院骨科,江西省南昌市330000 [2]南昌大学附属人民医院,江西省南昌市330000 [3]赣南医学院骨科,江西省赣州市341000

出　　处：《中国组织工程研究》2021年第4期621-625,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81560355),项目负责人:熊龙。

摘　　要：背景:如何募集内源性种子细胞促进成骨,为细胞提供适宜的骨再生微环境并保证新生骨血管化进程,是骨组织工程研究的难点。目的:综述多级微管结构骨支架载体促进成骨、成血管的作用。方法:由第一作者检索中国知网、万方、PubMed、Web of Science数据库1950至2020年有关多级微管结构骨支架载体促进成骨、成血管作用的文献,检索词为"多级微管结构、骨形成、血管生成;Hierarchical microchanneled scaffolds、osteogenesis、angiogenesis"。最终选取符合纳入标准的文献共计52篇。结果与结论:多级微管结构骨支架载体有良好的促进成骨、成血管作用,在骨组织工程中有较好的应用前景。目前多级微管结构骨支架载体在骨组织工程理论上比较成熟,体外实验证实了多级微管结构能较好地促进间充质干细胞黏附并可分化为成骨细胞系,但是体内实验成骨效果仍然欠佳,在成骨效果上仍然具有进一步提高的可能。将来的研究重点可能应该放在构建多级微管结构复合物,如载趋化因子或细胞因子可进一步提高体内实验的成骨效率。BACKGROUND:How to recruit endogenous seed cells to promote osteogenesis,provide appropriate bone regeneration microenvironment for cells and ensure the vascularization process of new bone are the difficulties of bone tissue engineering research.OBJECTIVE:To review the osteogenesis and vasculogenesis from the aspects of hierarchical microchanneled bone scaffold.METHODS:The first author searched relevant literature on the promoting effect of hierarchical microchanneled bone scaffold on osteogenesis and angiogenesis from China National Knowledge Infrastructure,Wanfang,PubMed and Web of Science databases from 1950 to 2020.The key words were“hierarchical microchanneled scaffolds,osteogenesis,and angiogenesis”in Chinese and English.Finally,a total of 52 articles meeting the inclusion criteria were selected.RESULTS AND CONCLUSION:Hierarchical microchanneled bone scaffold has a good role in promoting osteogenesis and vascularization,and has a good application prospect in bone tissue engineering.At present,hierarchical microchanneled bone scaffold is mature in the theory of bone tissue engineering.In vitro experiments have confirmed that the hierarchical microchanneled bone scaffold can promote the adhesion and differentiation of mesenchymal stem cells into osteoblasts,but the osteogenic effect in vivo is still poor,and it is still possible to further improve the osteogenic effect.In the future,we should focus on the construction of hierarchical microchanneled bone scaffold complexes,such as chemokines or cytokines,which can further improve the osteogenic efficiency in vivo.

关 键 词：骨 支架 材料 多级微管 成骨分化 新生血管 骨组织修复 巨噬细胞 免疫 

分 类 号：R459.9[医药卫生—治疗学] R-331[医药卫生—临床医学]