河南奶山羊线粒体D-loop区遗传多态性和遗传分化研究  

Genetic diversity and genetic differentiation analysis of mitochondrion D-loop region in Henan dairy goats

作　　者：韩浩园 权凯 闫志浩 高慧军 刘德奇 王先宁 尹慧茹 赵金艳 贾万里 李红霞 余世锋 王拥庆 李君 HAN Haoyuan;QUAN Kai;YAN Zhihao;GAO Huijun;LIU Deqi;WANG Xianning;YIN Huiru;ZHAO Jinyan;JIA Wanli;LI Hongxia;YU Shifeng;WANG Yongqing;LI Jun(School of Animal Science and Technology,Henan University of Animal Husbandry and Economics,Zhengzhou 450046,China;Xixia County Animal Husbandry Bureau,Nanyang 474500,China;Nanyang City Veterinary Drug Supervision Institute,Nanyang 473068,China;Nanyang Animal Husandry Bureau,Nanyang 473068,China;Shenqiu County Agricultural and Animal Husbandry Technology Research and Development Center,Zhoukou 466300,China;Henan Jianyang Animal Husbandry Co.,Ltd.,Nanyang 473000,China)

机构地区：[1]河南牧业经济学院动物科技学院,河南郑州450046 [2]西峡县畜牧局,河南南阳474500 [3]南阳市兽药监察所,河南南阳473068 [4]南阳市畜牧局,河南南阳473068 [5]沈丘县农牧科技研发中心,河南周口466300 [6]河南健羊牧业有限公司,河南南阳473000

出　　处：《河南农业大学学报》2020年第4期643-649,共7页Journal of Henan Agricultural University

基　　金：国家自然科学基金项目(31601915);河南牧业经济学院博士启动基金项目(2019HNUAHEDF021);河南省重点研发与推广专项(202102110237);河南牧业经济学院科研创新基金项目(XKYCXJJ2020013)。

摘　　要：为了解河南奶山羊遗传资源现状,对河南奶山羊、萨能奶山羊、关中奶山羊和伏牛白山羊线粒体DNA D-loop区全序列遗传多态性和遗传关系进行分析。结果表明,共存在16种单倍型,各品种单倍型多样度范围为0.391~0.814,核苷酸多样度的范围为0.00035~0.00464,核苷酸平均差异数范围为9.33333~13.66667,核苷酸岐异度变异范围为0.00833~0.01219,遗传分化系数范围为0.13565~0.36010,固定系数范围为0.58323~0.93550。山羊品种间系统发育树表明,河南奶山羊首先与伏牛白山羊聚为一支,其次河南奶山羊与萨能奶山羊聚类,最后与关中奶山羊聚类。系统发育树聚类结果与遗传距离分析、遗传分化分析结果相符,从遗传角度证实了河南奶山羊是以萨能奶山羊为父本,以河南地方品种为母本经杂交选育而成。To understand the current situation of the genetic resources of Henan dairy goats the genetic diversity of mitochondrion(mt DNA)D-loop and genetic relationship of Henan dairy goats,Saanen dairy goats,Guanzhong dairy goats,and Funiu white dairy goats were analyzed in this study.The results showed 16 haplotypes were identified based on D-loop variations.The haplotype diversity ranged from 0.391 to 0.814,the nucleotide diversity ranged from 0.00035 to 0.00464,the mean nucleotide difference ranged from 9.33333 to 13.66667,the nucleotide divergence variation ranged from 0.00833 to 0.01219,the genetic differentiation coefficient ranged from 0.13565 to 0.36010,and the fixed coefficient ranged from 0.58323 to 0.93550.The phylogenetic tree showed that Henan dairy goats clustered with Funiu white goats firstly,then they got together with Sannen dairy goats,and finally,they clustered with Guanzhong dairy goats.The results of phylogenetic tree corresponded with those of genetic distance and genetic differentiation analysis,verifying that Henan dairy goats were hybridized from Saanen as paternal line and domestic goats of Henan Province as maternal line.

关 键 词：线粒体DNA D-loop 奶山羊 遗传多样性 遗传距离 

分 类 号：S855.99[农业科学—临床兽医学]