雌激素对消化性溃疡模型小鼠组织损伤的影响及相关机制研究  

Study on the effect of estrogen on tissue damage in peptic ulcer model mice and its related mechanism

作　　者：郭艳红 姚青林[2] 张雪丽[1] 靳斌[1] GUO Yan-hong;YAO Qing-lin;ZHANG Xue-li;JIN Bin(Department of Gastroenterology,1.Xi'an First Hospital,Xi'an 710002;Tangdu Hospital,Xi'an 710038,Shaanxi,China)

机构地区：[1]西安市第一医院消化内科,陕西西安710002 [2]唐都医院消化内科,陕西西安710038

出　　处：《川北医学院学报》2020年第4期555-558,567,共5页Journal of North Sichuan Medical College

基　　金：陕西省自然科学基金(2015JQ4044)。

摘　　要：目的:探讨雌激素对消化性溃疡模型小鼠组织损伤的影响及作用机制。方法:36只健康C57BL/6雌性小鼠适应性饲养7 d后随机分为假手术对照组、假手术+PU模型对照组、假手术+PU模型+雌激素干预组、去势对照组、去势+PU模型对照组、去势+PU模型+雌激素干预组,每组各6只。根据实验设置分别建立消化性溃疡模型以及进行卵巢去势手术,雌激素干预组注射500μg/kg雌激素,对照组注射等量生理盐水。干预7 d后,分别检测并计算胃溃疡指数和溃疡抑制率、测定小鼠胃液和十二指肠液pH以及胃蛋白酶活性、试剂盒检测各组小鼠血清中活性氧(ROS)和谷胱甘肽过氧化物酶(GSH-PX)活性以及丙二醛(MDA)的含量、小鼠ELISA试剂盒检测小鼠血清中IL-17和IL-25含量。结果:与假手术对照组相比,假手术+PU模型对照组的溃疡指数、出血点数、ROS活性、MDA含量以及IL-17和IL-25含量均显著增加,胃液pH、十二指肠液pH、GSH-Px活性均显著降低(P<0.05);与去势对照组相比,去势+PU模型对照组的变化趋势与上述一致,差异也均具有统计学意义(P<0.05);而分别与假手术+PU模型对照组和去势+PU模型对照组相比,两个雌激素干预组以上所有指标变化趋势与上述相反(P<0.05)。与去势假手术组相比,去势组溃疡指数、出血点数、ROS活性、MDA含量以及IL-17和IL-25含量均显著增加,胃液pH、十二指肠液pH、GSH-Px活性均显著降低(P<0.05)。结论:采用雌激素治疗可显著减少消化性溃疡小鼠溃疡指数和出血点数,进而使得小鼠胃液和十二指肠液pH升高,促进消化性溃疡小鼠症状的改善,其作用机制可能与改善氧化应激和炎性相关指标有关。Objective:To investigate the effect of estrogen on tissue damage of peptic ulcer model mice and its related mechanism.Methods:Healthy C57BL/6 female mice were selected and divided into sham surgery control group,sham surgery+PU control group,sham surgery+PU+E2 group,castration control group,castration+PU control group,castration+PU In the E2 group,6 cases in each group.A peptic ulcer model and ovarian castration surgery were established according to the experimental settings.The estrogen intervention group was injected with 500μg/kg estrogen,and the control group was injected with the same amount of normal saline.After 7 days of intervention,gastric ulcer index,ulcer inhibition rate,pH and pepsin activity of gastric juice and duodenal fluid were measured,and the activities of reactive oxygen species(ROS),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)in serum were detected by kit,and the contents of IL-17 and IL-25 in serum were detected by ELISA kit.Results:Compared with the sham operation group,the ulcer index,bleeding points,ROS activity,MDA content,and IL-17 and IL-25 contents in the sham operation+PU group increased significantly.The gastric juice pH,duodenal fluid pH and GSH-Px activities were significantly reduced(P<0.05).Compared with the castration control group,the change trend of the castration+PU group was consistent with the above,and the differences were also statistically significant(P<0.05),compared with the sham surgery+PU control group and the castration+PU control group,respectively,the changes in all indicators of the two E2 treatment groups were opposite to those described above(P<0.05).Compared with the castration sham operation group,the ulcer index,bleeding points,ROS activity,MDA content,and IL-17 and IL-25 content in the castration group were significantly increased,and the gastric juice pH,duodenal juice pH,and GSH-Px activity were significantly reduced(P<0.05).Conclusion:Treatment with estrogen can significantly reduce the ulcer index and bleeding points of peptic ulcer mice,wh

关 键 词：消化性胃溃疡 雌激素 溃疡指数 活性氧 胃蛋白酶 

分 类 号：R96[医药卫生—药理学]