基于近红外技术的沼液丁酸和异丁酸定量分离研究  

Separation of Butyric Acid and Iso butyric Acid in Biogas Slurry by NIRS

作　　者：贺莉 冉毅 李冰峰 HE Li;RAN Yi;LI Bing-feng(Biogas Institute of Ministry of Agriculture and Rural Affairs, Ministry of Agriculture and Rural Affairs, Chengdu 610041,China;Rural Energy and Environment Agency, Ministry of Agriculture and Rural Affairs, Beijing 100125,China)

机构地区：[1]农业农村部沼气科学研究所,成都610041 [2]农业农村部农业生态与资源保护总站,北京100125

出　　处：《中国沼气》2020年第4期9-12,共4页China Biogas

基　　金：四川省科技支撑项目(2018JY0543);国家自然科学基金青年项目(31922208);中国农业科学院科技创新工程(CAAS-ASTIP-2016-BIOMA)。

摘　　要：沼液中挥发酸的浓度是制约厌氧发酵进程的关键因素。在采用HPLC对挥发酸分离时,丁酸和异丁酸这两种同分异构体存在色谱峰重叠无法准确定量的问题。文章利用Bruker MPA多功能型傅立叶变换近红外光谱仪在4000~12000 nm光谱范围对丁酸和异丁酸混合溶液进行定量分离,丁酸和异丁酸的校正模型外部验证的RMSE均为3.862%,MAPE小于9%。实验结果表明,近红外光谱技术能实现丁酸和异丁酸在HPLC中的快速定量分析,可节省检测时间和降低检测成本。The concentration of volatile acids in biogas slurry is the key factor restricting the anaerobic fermentation process.In the separation of volatile acids by HPLC,butyric acid and isobutyric acid are two isomers with overlapping peaks,which can not be accurately quantified.In this paper,Bruker MPA multi-functional Fourier transform near infrared spectrometer was used to quantitatively separate butyric acid and isobutyric acid in the spectral range of 4000~12000 nm.The calibration model of butyric acid and isobutyric acid was verified by external validation with RMSE was 3.862%and MAPE was less than 9%.The experimental results showed that NIR could realize the rapid quantitative analysis of butyric acid and isobutyric acid in HPLC,which could save detection time and reduce detection cost.

关 键 词：沼液 丁酸 异丁酸 近红外 

分 类 号：S216.4[农业科学—农业机械化工程] X713[农业科学—农业工程]