复方益肾解毒汤对大鼠急性肾缺血再灌注损伤的机制研究  

Protective Effect and Mechanism of Compound Yishen Jiedu Decoction on Acute Renal Ischemia-Reperfusion Injury in Rats

作　　者：贾跃军 左文仁 苏学林 汤井源 何垚澍 王世衍 苏健[2] 周恩超[2] 朱清毅[2] JIA Yue-jun;ZUO Wen-ren;SU Xue-lin;TANG Jing-yuan;HE Yao-shu;WANG Shi-yan;SU Jian;ZHOU En-chao;ZHU Qing-yi(Department of Urology Surgery, Zhenjiang Hospital of Chinese Traditional and Western Medicine, Zhenjiang, 212000, China;The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing, 210029, China)

机构地区：[1]镇江市中西医结合医院泌尿外科,江苏镇江212000 [2]南京中医药大学附属医院/江苏省中医院,江苏南京210029

出　　处：《南京中医药大学学报》2020年第4期494-497,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金青年基金(81902570)。

摘　　要：目的探讨益肾解毒汤对大鼠急性肾缺血再灌注的保护作用及机制研究。方法48只雄性大鼠随机分为4组:假手术组、肾缺血再灌注组(RIR组)、益肾解毒汤+肾缺血再灌注组(Drug+RIR组)、姜黄素+肾缺血再灌注组(Cur+RIR组)。术后24、96 h取大鼠目内眦采血,比较各组大鼠血清中肌酐(Scr)、尿素氮(BUN)水平;取术后24 h大鼠肾脏组织,比较各组肾脏损伤评分;比较假手术组、RIR组和Drug+RIR组3组炎症因子NF-κB水平。结果与假手术组比较,RIR组、Drug+RIR组和Cur+RIR组血清Scr、BUN、肾小管损伤评分均升高,Drug+RIR组高于Cur+RIR组,但均明显低于RIR组(P<0.05);且比较术后96 h RIR组、Drug+RIR组2组血清Scr、BUN,Drug+RIR组肾功能指标恢复更快。结论复方益肾解毒汤对大鼠肾脏缺血再灌注损伤有明显保护作用,其机制可能与NF-κB相关炎症因子的表达有关。OBJECTIVE To investigate the protective effect and mechanism of compound Yishen Jiedu decoction on acute renal ischemia-reperfusion injury in rats.METHODS 48 male rats were randomly divided into four groups:sham surgery group,renal ischemia-reperfusion group(RIR group),Yishen jiedu decoction plus renal ischemia-reperfusion group(Drug+RIR group),and curcumin plus renal ischemia-reperfusion group(Cur+RIR group).Blood samples were collected from the fundus vein at 24 h and 96 h after surgery to compare the serum levels of creatinine(Scr)and urea nitrogen(BUN)in each group.The renal tissues were collected 24 h after surgery,and the renal injury scores of each group were compared.NF-κB levels in Sham surgery group,RIR group and Drug+RIR group were compared.RESULTS Compared with the sham surgery group,serum Scr,BUN,and renal tubular injury scores in RIR group,Drug+RIR group and Cur+RIR group were all increased.Serum Scr,BUN and renal injury scores of the Drug+RIR group were higher than those of the Cur+RIR group,but were significantly lower than those of the RIR group(P<0.05).And compared the serum Scr and BUN of 96h affer surgery,the renal function of the Drug+RIR group recovered faster than RIR group.CONCLUSION Compound Yishen Jiedu decoction has obvious protective effect on renal ischemia-reperfusion injury in rats,and its mechanism may be related to NF-κB related inflammatory factors.

关 键 词：益肾解毒汤 肾缺血再灌注损伤 NF-ΚB 加速康复 

分 类 号：R285.5[医药卫生—中药学]