基于毒素多肽BmK NaTx-13的全蝎质控工艺研究  

Development of a BmK Scorpion Quality Control Technology Based on Toxin Peptide BmK NaTx-13

作　　者：陈永根 桑明 卢悟广[3,4] 王明远 刘金满 陈胜君 李松 胡春萍[3,4] 王奇[1,2] 曹鹏 CHEN Yong-gen;SANG Ming;LU Wu-guang;WANG Ming-yuan;LIU Jin-man;CHEN Sheng-jun;LI Song;HU Chun-ping;WANG Qi;CAO Peng(Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China;Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China;Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210028, China;School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China;Jiangsu Key Laboratory of Key Technology for Preparation and Quality Control of Traditional Chinese Medicine Formula Particles, Jiangyin Tianjiang Pharmaceutical Co., Ltd, Wuxi, 214400, China)

机构地区：[1]广州中医药大学科技创新中心,广东广州510405 [2]广州中医药大学临床药理研究所,广东广州510405 [3]南京中医药大学附属中西医结合医院,江苏南京210028 [4]南京中医药大学药学院,江苏南京210023 [5]江阴天江药业有限公司,江苏省中药配方颗粒制备与质量控制关键技术重点实验室,江苏无锡214400

出　　处：《南京中医药大学学报》2020年第4期509-516,共8页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金优秀青年基金(81622048);广州市科技局项目(201805010005)。

摘　　要：目的基于毒素多肽建立一种新型的全蝎质量检测工艺。方法从东亚钳蝎毒液中分离得到了一种新型钠通道毒素BmK NaTx-13,制备并鉴定了BmK NaTx-13的特异性抗体,并通过竞争性ELISA法检测全蝎提取物中的BmK NaTx-13的含量。结果建立的竞争性酶联免疫吸附法可快速检测全蝎提取物中的BmK NaTx-13的含量并呈现良好的浓度线性依赖。结论该研究为全蝎质控提供了一种快速免疫检测方法,为提升全蝎入药质量标准提供了借鉴。OBJECTIVE To develop a BmK Scorpion quality control technology based on toxin peptide.METHODS In this study,a novel sodium channel toxin BmK NaTx-13 was isolated from BV,and the specific polyclonal antibody against BmK NaTx-13 was obtained by immunizing rabbits.RESULTS A competitive enzyme linked immunosorbent assay(ELISA)based on BmK NaTx-13 specific antibody could detect the content of BmK NaTx-13 in the processed BmK scorpion extract and showed a good linear concentration dependent.CONCLUSION This research provided a rapid immunoassay method for establishing the quality detection processed BmK scorpion,and provided an insight for improving the quality standard of whole scorpion medicine.

关 键 词：全蝎 BmK NaTx-13 ELISA 质控工艺 

分 类 号：R284[医药卫生—中药学]