鼓槌石斛多糖提取工艺优化及含量测定  

Determination of polysaccharide content and Extraction Technology from Dendrobiun chrysotoxum

作　　者：滕建北[1] 张婷[2] 刘喜华[2] 刘安韬[2] 莫展雄 Teng Jianbei;Zhang Ting;Liu Xihua;Liu Antao;Mo Zhanxiong(Guangxi University of traditional Chinese medicine Nanning,530001;Guangxi Vocational College of health,Nanning,530023)

机构地区：[1]广西中医药大学,南宁530001 [2]广西卫生职业技术学院,南宁530023

出　　处：《北方药学》2020年第7期8-10,共3页Journal of North Pharmacy

基　　金：2018年中药特色技术传承人才培训项目(编号:T20184828005);广西壮瑶药重点实验室项目“壮药小黄草全成分颗粒的制备研究”(GXZYZZ201707);广西中医药大学课题“广西产石斛的质量评价及其多糖提取工艺研究”。

摘　　要：目的:优化鼓槌石斛多糖提取工艺,并测定其含量。方法:通过单因素和正交实验,考察提取时间、料液比和提取次数等影响因素,研究鼓槌石斛最佳的提取工艺参数。参照《中国药典》2015版石斛多糖含量测定,采用硫酸-苯酚显色法,测定药材多糖含量。结果:确定最佳提取工艺为料液比为1∶100,提取时间1.5 h,提取2次,测得鼓槌石斛多糖含量30.65%。结论:该方法稳定、可靠,可作为鼓槌石斛多糖的提取方法。Objective:Optimum extraction process and determination of polysaccharide content of Dendrobiun chrysotoxum. Methods:Extraction time,Material-liquid ratio and extraction times polysaccharide content was determined by single factor investigation and orthogonal experiment. Phenol-sulfuric acid method was used to quantitative determination. Results:The ratio of material to liquid was 1:100,and the extraction time was 1.5 h. The extraction times were 2 times,and the content of polysaccharide in Dendrobiun chrysotoxum was 30.65% under these conditions. Conclusion:It is stable and reliable method that can be used to extract and determine polysaccharides of Dendrobiun chrysotoxum.

关 键 词：鼓槌石斛 多糖 提取工艺 正交试验 硫酸-苯酚法 

分 类 号：R284[医药卫生—中药学]