促生细菌通过miRNA调节拟南芥根部关键基因的表达  

Growth Promoting Bacteria Regulate the Expression of Key Genes in theRoots of Arabidopsis thaliana Through miRNAs

作　　者：李菲[1,2] 龚记熠 张习敏 张宇斌[1,2] 乙引[1,2] Li Fei;Gong Jiyi;Zhang Ximin;Zhang Yubin;Yi Yin(Key Laboratory of Biodiversity Conservation of Southwest China Karst Mountain,School of Life Sciences,Guizhou Normal University,Guiyang,550001;Guizhou Key Laboratory of Plant Physiology and Development Regulation,School of Life Sciences,Guizhou Normal University,Guiyang,550001)

机构地区：[1]贵州师范大学生命科学学院,西南喀斯特山地生物多样性保护国家林业局重点实验室,贵阳550001 [2]贵州师范大学生命科学学院,贵州省植物生理与发育调控重点实验室,贵阳550001

出　　处：《分子植物育种》2020年第17期5690-5699,共10页Molecular Plant Breeding

基　　金：国家自然科学基金委员会-贵州省人民政府喀斯特科学研究中心项目(U1812401)资助。

摘　　要：促生细菌在生态和农业领域的应用前景,已经引起了广泛关注,但目前对促生细菌对植物生理影响的分子机理的研究还比较少。miRNA被视为植物中重要的一类转录后调控因子,通常以转录因子为靶标,并参与多种生理过程的调节。本研究发现在拟南芥主根促生细菌枯草芽孢杆菌ACCC 01380处理下,长度明显高于普通细菌大肠杆菌处理;采集两种处理下拟南芥植株根部样本,进行小RNA测序,比较两种处理下拟南芥根部miRNA表达情况;筛选出表达受到促生细菌枯草芽孢杆菌ACCC 01380影响的miRNA;利用拟南芥丰富的生物信息数据,预测两种处理下差异表达miRNA的靶基因,分析其功能;最后对差异表达miRNA及其靶基因进行实时定量PCR验证。本研究为揭示促生细菌对植物生理影响的分子机理,以及这一过程中miRNA的作用提供了理论基础。The application potential of plant growth-promoting bacteria in environment restoration and agriculture have attracted the attention of scientists.However,the molecular mechanism of growth-promoting bacteria on plant physiology has not been thoroughly studied.miRNAs are important post-transcriptional regulatory factors in plants,which usually target transcription factors and participate in the regulation of various physiological processes.This study found that,under the treatment of plant growth-promoting bacterium Bacillus subtilis ACCC 01380,the length of the main root of Arabidopsis thaliana was significantly longer than that of the common bacterium E.coli;The root samples of Arabidopsis plants under two treatments were collected,and small RNA sequencing was performed to compare the miRNA profiling under two treatments;Arabidopsis root miRNAs whose expression is affected by bacterium Bacillus subtilis ACCC 01380 were screened;Making use of the rich biological information of Arabidopsis thaliana,target genes of differentially expressed miRNAs under the two treatments were predicted,and their function was analyzed;Finally,the differentially expressed miRNAs and their target genes were verified by real-time quantitative PCR.This study provides a theoretical basis for revealing the molecular mechanism of the effects of growth-promoting bacteria on plant physiology and the role of miRNA in this process.

关 键 词：促生细菌 拟南芥 MIRNA 靶基因 

分 类 号：Q943.2[生物学—植物学]