金鱼草AmPIN1a基因过表达载体的构建及转基因分析  

Transgenic Analysis and Construction of an Overexpression Vector for theAmPIN1a Gene in Antirrhinum majus

作　　者：王松[1] 陈友根[1] 王瑞生 李委[1] 甘德芳[1] 曹舸洋 王冬良[1] Wang Song;Chen Yougen;Wang Ruisheng;Li Wei;Gan Defang;Cao Geyang;Wang Dongliang(College of Horticulture,Anhui Agricultural University,Hefei,230036)

机构地区：[1]安徽农业大学园艺学院,合肥230036

出　　处：《分子植物育种》2020年第17期5738-5747,共10页Molecular Plant Breeding

基　　金：安徽省重点研究与开发计划项目(1704e0302200);新疆皮山县设施蔬菜新品种引进及绿色高效种植技术集成与示范(1804e03020325)共同资助。

摘　　要：本研究以金鱼草的AmPIN1a为目的基因,AmPIN1a基因全长CDS为1 836 bp,通过热激法(电击发)将构建过表达载体pCAMBIA1302 (1301)-AmPIN1a导入根癌农杆菌GV3101中,采用叶盘法转化拟南芥,观察其表型并用外源IAA对其进行处理。结果表明,转化AmPIN1a基因的拟南芥突变体在0~12 d内主根长度略短于野生型主根长度,而且侧根发育明显增多。移栽后,AmPIN1a拟南芥突变体的前期叶片大小也小于野生型,24 d后叶片逐步超过野生型,花序轴萌发晚,但发育较快。过表达AmPIN1a基因的拟南芥在外缘IAA处理下表现出比野生型拟南芥更敏感的性状,由此推测AmPIN1a基因可能在生长素运输过程中起作用。This experiment regarding the AmPIN1a gene of Snapdragon as the target gene.The full-length CDS of AmPIN1a gene is 1836 bp,we overexpressed AmPIN1a in Arabidopsis thaliana and treated transformants with IAA.12 days after treatment,the main root of AmPIN1a overexpressed transformants were slightly shorter than that of wild type.And lateral root development increased significantly.After transplanting,the leaf size of AmPIN1a over-expressed transformants was also smaller than that of wild type at the early stage.After 24 days,the leaf size gradually exceeded that of the wild type.Late germination of inflorescence axis,but has rapid development.Under exogenous IAA treatment,Arabidopsis over-expressing AmPIN1a gene was found to be more susceptible to IAA stimulation than wild-type Arabidopsis,so we can speculate that this gene may play a significant role in the auxin transport.

关 键 词：金鱼草(Antirrhinum majus) 叶序发育 PIN基因 AmPIN1a IAA 

分 类 号：Q943.2[生物学—植物学] S682.19[农业科学—观赏园艺]