蛋白质对蒙脱石基药物缓释及其生物效应影响  

Effect of Protein on Sustained Release of Montmorillonite-based Drugs and Its Biological Effects

作　　者：潘璧莹 魏均曼 夏觅真 吴琳梅 PAN Bi-ying;WEI Jun-man;XIA Mi-zhen;WU Lin-mei(College of Life Science,Anhui Medical University,Hefei Anhui 230032,China)

机构地区：[1]安徽医科大学生命科学学院,安徽合肥230032

出　　处：《广州化工》2020年第17期40-43,共4页GuangZhou Chemical Industry

基　　金：安徽医科大学博士科研基金项目(No:XJ201515)。

摘　　要：实验制备5-氟尿嘧啶/蒙脱石缓释药物(5-Fu/Mt),研究5-Fu/Mt缓释药物与蛋白质的相互作用及其对体外培养肺癌A549细胞的影响。研究表明:牛血清白蛋白(BSA)在5-Fu/Mt上的吸附等温曲线呈S型,且10 h后达到吸附/脱附平衡;随着BSA浓度的增加,缓释药物中5-Fu的释放量减小;5-Fu/Mt对体外培养肺癌A549细胞的半数抑制浓度为27.67 ug/mL,高浓度BSA的存在会减弱5-Fu/Mt对肺癌A549细胞生长的抑制作用。The 5-fluorouracil/montmorillonite sustained-release drugs(5-Fu/Mt)were prepared.Besides,the interaction between protein and 5-Fu/Mt and the resulted effect on A549 cells cultured in vitro were analyzed.The results showed that the adsorption isotherm of bovine serum albumin(BSA)on 5-Fu/Mt showed an S-shaped isotherm,and the adsorption/desorption equilibrium was reached after 10 h.The cumulative drug release of 5-Fu from 5-Fu/Mt decreased with increasing BSA concentration.In vitro cell culture experiment results revealed that the half inhibitory concentration of 5-Fu/Mt on lung cancer A549 cells was 27.67μg/mL,and the presence of high concentration of BSA weakened the growth inhibitory effect of 5-Fu/Mt on A549 cells.

关 键 词：蒙脱石 蛋白质 缓释 5-氟尿嘧啶 生物效应 

分 类 号：R945[医药卫生—微生物与生化药学]