脊髓损伤对下丘脑c-Fos表达及神经细胞凋亡的影响  

Effect of spinal cord injury on the expression of c-Fos in hypothalamus and neuronal apoptosis

作　　者：孙鹏[1] 叶祥明[1] 田亮[1] 李厥宝[1] 程瑞动[1] SUN Peng;YE Xiangming;TIAN Liang;LI Juebao;CHENG Ruidong(Department of Rehabilitation Medicine,Zhejiang Provincial People's Hospital,People's Hospital of Hangzhou Medical College,Hangzhou 310014,China)

机构地区：[1]浙江省人民医院杭州医学院附属人民医院康复医学科,浙江杭州310014

出　　处：《中国现代医生》2020年第21期45-48,53,F0003,共6页China Modern Doctor

基　　金：国家自然科学基金青年基金(81601965);浙江省医药卫生科技计划(2020KY435)。

摘　　要：目的探讨脊髓损伤后下丘脑神经细胞凋亡情况,确认脊髓损伤导致大脑损害的特点及其在相关并发症发生发展中的可能机制。方法18只SD大鼠随机分为两组:假模组(Sham组)和脊髓损伤组(SCI组),以改良的Allen’s撞击法制作脊髓损伤动物模型,常规饲养,分别于8周取大鼠下丘脑组织,应用免疫荧光染色法观察脊髓损伤大鼠下丘脑组织c-Fos表达,TUNEL法观察两组下丘脑神经细胞凋亡情况,应用ELISA法检测两组大鼠下丘脑炎症因子(TNF-α、IL-1β、IL-6)表达。结果脊髓损伤后8周,SCI组较Sham组下丘脑c-Fos表达明显增加(P<0.05),同时下丘脑可见明显凋亡细胞,主要表现为胞浆减少,细胞核深染、固缩在一边;SCI组TUNEL阳性细胞数比Sham组明显增多,差异有统计学意义(P<0.01);SCI组大鼠下丘脑包括TNF-α、IL-1β、IL-6等多种炎症因子水平较Sham组明显升高,差异有统计学意义(P<0.01)。结论脊髓损伤可对下丘脑造成损害,表现为下丘脑神经细胞凋亡及炎症反应明显增加,其可能与c-Fos表达有关,脊髓损伤后对下丘脑的损害可能是某些并发症发生发展的发病机制。Objective To investigate the neuronal apoptosis of hypothalamus after spinal cord injury,and to confirm the characteristics of spinal cord injury leading to brain damage and its possible mechanism in the occurrence and development of related complications.Methods 18 SD rats were randomly divided into two groups including sham module(Sham group)and spinal cord injury group(SCI group).The animal model of spinal cord injury was made by modified Allen's impact method.And the rats were conventionally fed.Rat hypothalamus tissues were taken at the 8th week.The expression of c-Fos in the hypothalamus of spinal cord injury rats was observed by immunofluorescence staining.The neuronal apoptosis of hypothalamus in the two groups was observed by TUNEL method.The expressions of hypothalamic inflammatory factors(TNF-α,IL-1β,IL-6)of two groups were detected by ELISA method.Results Eight weeks after spinal cord injury,the expression of c-Fos in the hypothalamus of the SCI group was significantly higher than that of the Sham group(P<0.05).At the same time,there were obvious apoptotic cells in the hypothalamus.The main manifestations werecytoplasm reduction,nucleus deep staining and shrinkage of the nucleus on one side.The number of TUNEL positive cells in the SCI group was significantly higher than that in the Sham group(P<0.01).The levels of various inflammatory factors in the hypothalamus of the SCI group,including TNF-α,IL-1β,IL-6,were higher than those in Sham group,and the difference between the two groups was statistically significant(P<0.01).Conclusion Spinal cord injury can cause damage to the hypothalamus,which is manifested by a marked increase in neuronal apoptosis and inflammatory response.This may be related to the expression of c-Fos.The damage of spinal cord injury to the hypothalamus may be the pathogenesis of the development of certain complications.

关 键 词：脊髓损伤 下丘脑 C-FOS 神经细胞凋亡 炎症反应 

分 类 号：R651.2[医药卫生—外科学]