养分增加对蓝莓根部真菌群落的影响  

Effect of Nutrient Increase on Fungal Communities Associated with Blueberry Hair Roots

作　　者：何逸菲 唐卓菲 黄昕怡 颜劲 吴朱杰 KABORE Marthur Fabrice 孙立夫[1] 张艳华[1] HE Yifei;TANG Zhuofei;HUANG Xinyi;YAN Jin;WU Zhujie;KABORE Marthur Fabrice;SUN Lifu;ZHANG Yanhua(School of Life Science,Shaoxing University,Shaoxing,Zhejiang 312000)

机构地区：[1]绍兴文理学院生命科学学院,浙江绍兴312000

出　　处：《绍兴文理学院学报》2020年第8期85-90,共6页Journal of Shaoxing University

基　　金：浙江省自然科学基金项目“云锦杜鹃根部真菌群落结构动态对伴生种变化的响应”(LY19C030002);绍兴市科技局项目“菌根修复在镜湖湿地重金属污染土壤中的应用”(2017B70061);浙江省大学生科技创新项目“养分增加对蓝莓根部真菌群落动态影响的研究”(2019R432021).

摘　　要：杜鹃花科(Ericaceae)植物在贫瘠的土壤中占优势,与其特殊的菌根共生体有关.本文以蓝莓(Vaccinium sp.)为对象,研究土壤养分增加对蓝莓根部真菌群落组成和结构的影响.在不同浓度梯度的N、P和凋落物单独和混合培养4个月后,收集毛根和土壤样品,通过高通量测序,明确其根部真菌群落的组成和结构,通过ICP-AES法和元素分析法测量土壤P、K、Mg、Mn、Cu、C和N含量.结果表明:蓝莓根部真菌主要来自Ascomycota和Basidiomycota;添加凋落物的处理组,其蓝莓根部真菌丰富度显著高于添加N和P的各处理组,且公认的杜鹃花类菌根(ERM)真菌Oidiodendron maius与Mn的含量呈显著的正相关关系,而Sebacinales则与包括P在内的多个元素含量呈负相关关系.土壤N和P养分增加对功能性真菌在蓝莓根部的建立有不利影响.Ericaceae plants,dominant in poor soil,are considered to be related to their special mycorrhizal symbionts.In this paper,the effect of soil nutrients on the composition and structure of fungal communities in blueberry hair roots was studied.After 4 months of separate and mixed culture of N,P and litter with different concentration gradients,the samples of the hair roots and soil were collected,and the changes of fungal community composition and structure associated with blueberry roots were determined by high-throughput sequencing.The contents of P,K,Mg,Mn and Cu in soil were measured by ICP-AES,and the contents of C and N were measured by elemental analysis.The results show that the fungi associated with blueberry roots mainly come from Ascomycota and Basidiomycota;under the treatment group with litter only,the fungal richness is significantly higher than that in the treatment groups with N or P,and the putative ericoid mycorrhizal(ERM)fungi,Oidiodendron maius,show a significantly positive correlation with Mn contents,while Sebacinales has a significantly negative correlation with multiple elements including P contents.The increase of soil nutrients will be responsible for the establishment of functional fungi in blueberry hair roots.

关 键 词：蓝莓 根部真菌 群落 氮 磷 凋落物 

分 类 号：Q938[生物学—微生物学]