蛴螬对兔光损伤视网膜变性Caspase-8、Bax、Bid、Caspase-3 mRNA表达的影响  

Effects of Grub Extract on mRNA Expressions of Caspase-8,Bax,Bid and Caspase-3 in Rabbits with Optical Damage and Retinal Degeneration

作　　者：蒋鹏飞[1,2] 彭俊 吴大力 彭清华[1,2,3] JIANG Pengfei;PENG Jun;WU Dali;PENG Qinghua(Hunan University of Chinese Medicine,Changsha 410208,China;Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Eye,Ear,Nose and Throat Diseases in Hunan Province,Changsha 410208,China;The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]中医药防治眼耳鼻咽喉疾病湖南省重点实验室,湖南长沙410208 [3]湖南中医药大学第一附属医院,湖南长沙410007 [4]广西中医药大学第一附属医院,广西南宁530023

出　　处：《中医药信息》2020年第5期1-4,共4页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81804150);湖南省中医药科研基金资助项目(2010012);湖南省教育厅科研基金资助项目(09C729);湖南中医药大学科研基金资助项目(3601010157);中医药防治五官科疾病湖南省重点实验室建设项目(2017TP1018);中央财政支持地方高校建设项目;国家中医药管理局中医眼科学重点学科建设项目;湖南省中医五官科学重点学科建设项目;湖南省研究生科研创新项目(CX2018B497);湖南省自然科学基金资助项目(2019JJ40226)。

摘　　要：目的:探讨蛴螬提取物对兔光损伤视网膜变性Caspase-8、Bax、Bid、Caspase-3 mRNA表达的影响。方法:将30只雄性实验性兔随机平均分为空白组、模型组、蛴螬低剂量组、蛴螬中剂量组和蛴螬高剂量组。除空白组外,其余4组均建立光损伤视网膜变性模型,在给药14 d处死后取材采用RT-qPCR法测定Caspase-8 mRNA、Bax mRNA、Bid mRNA、Caspase-3 mRNA的表达水平。结果:与模型组比较,蛴螬低、中、高剂量组的Caspase-8 mRNA、Bax mRNA、Bid mRNA、Caspase-3 mRNA相对表达量降低,差异具有统计学意义(P<0.05);与蛴螬低剂量组比较,中、高剂量组的Bax mRNA和Caspase-3 mRNA改善更为明显(P<0.05),但中、高剂量组之间没有统计学意义(P>0.05)。结论:蛴螬通过抑制Caspase-8 mRNA、Bax mRNA、Bid mRNA、Caspase-3 mRNA的表达,抑制细胞凋亡受体信号通路来发挥保护视网膜作用,中、高剂量组效果较好。Objective:To investigate the effects of grub extract on the mRNA expressions of Caspase-8,Bax,Bid and Caspase-3 in rabbits with optical damage and retinal degeneration.Methods:30 experimental rabbits were randomly divided into the blank group,the model group,the grub groups of low-dose,medium-dose and high-dose,with 6 rabbits(12 eyes)in each group.The model of optical damage and retinal degeneration was established.The mRNA expressions of Caspase-8,Bax,Bid and Caspase-3 were determined by RT-qPCR after 14 days of intervention.Results:Compared to the model group,the relative mRNA expressions of Caspase-8,Bax,Bid and Caspase-3 were significantly increased in the grub groups of low-dose,medium-dose and high-dose(P<0.05).Compared to low-dose group,the improvements of Bax mRNA and Caspase-3 mRNA were more significant in the medium-group and high-dose group(P<0.05);of which there were no statistical differences between the medium-dose group and the high-dose group(P>0.05).Conclusion:Grub extraction can inhibit the mRNA expressions of Caspase-8,Bax,Bid and Caspase-3 and inhibit the apoptotic receptor signaling pathway to protect retina.The groups of medium-dose and high-dose have better effects.

关 键 词：蛴螬提取物 光损伤 CASPASE-8 BAX BID Caspase-3 

分 类 号：R285.5[医药卫生—中药学]