引火汤改善长期OVX小鼠焦虑样行为的机制研究  

Mechanism of Yinhuo Decoction on Improving Anxiety-Like Behavior in Long-Term OVX Mice

作　　者：伊金月 张悦[1] 邱琦 刘爽[1] 关雨佳 张宁[1,2] 孙慧峰[1] 董晓红[2] 徐红丹[1,2] YI Jinyue;ZHANG Yue;QIU Qi;LIU Shuang;GUAN Yujia;ZHANG Ning;SUN Huifeng;DONG Xiaohong;XU Hongdan(Heilongjiang University of Chinese Medicine,Harbin 150040,China;College of Jiamusi,Heilongjiang University of Chinese Medicine,Jiamusi 154007,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007

出　　处：《中医药信息》2020年第5期27-32,共6页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81673581);黑龙江省自然科学基金项目(H2018058);黑龙江中医药大学科研基金基金(2018pt04,2018jkcy05,2015xy04);黑龙江中医药大学工程中心(2017SEC02)。

摘　　要：目的:探讨引火汤治疗长期双侧卵巢摘除术(OVX)小鼠的焦虑样行为的机制。方法:将40只ICR小鼠随机分为假手术组(Sham组)、模型组(OVX组)、雌二醇组(E 2组)和引火汤组(YHT),采用双侧卵巢摘除联合单频超声刺激复制长期OVX焦虑模型,采用明暗箱(LDB)和高架十字迷宫(EPM)观察小鼠的焦虑样行为,检测各组小鼠脑内雌激素水平,测量体质量、子宫系数,HE染色观察子宫形态,尼氏染色观察海马神经元状态。结果:与OVX组相比,YHT组小鼠焦虑样行为显著改善,脑内雌激素水平显著增加,海马神经元形态完好,子宫形态未见改变。结论:引火汤能改善长期OVX小鼠的焦虑样行为,其作用机制可能是发挥雌激素效应并对ERβ有选择性激活作用。Objective:To investigate the mechanism of Yinhuo Decoction in treating long-term anxiety-like behavior in OVX mice.Methods:40 ICR mice were randomly divided into the sham operation group,the model group(OVX),estradiol(E 2)group and Yinhuo Decoction group.The bilateral ovarian ablation combined with single-frequency ultrasound stimulation was used for long-term OVX replication.LDB and EPM tests were used to detect anxiety-like behaviors.The level of estrogen in the brain was measured.The body weight and uterus coefficient were measured.The morphology of the uterus was observed by HE staining.The hippocampal neuron status was observed by Nissl staining.Results:Compared to the OVX group,the anxiety-like behavior of Yinhuo Decoction group was significantly improved,the level of estrogen in the brain was significantly increased,the morphology of the hippocampal neurons was intact,and the morphology of the uterus was unchanged.Conclusion:Yinhuo Decoction can improve the anxiety-like behavior of long-term OVX mice.The mechanism of action may be related to exerting estrogenic effects and selectively activating ERβ.

关 键 词：引火汤 OVX 焦虑症 雌激素 ERΒ 

分 类 号：R285.5[医药卫生—中药学]