显脉金花茶叶片中山奈酚的提取分离及含量测定  

Extraction separation and determination of kaempferol in Camellia euphlebia Merr.ex Sealy

作　　者：陈秋虹 刘鹏 韦英亮 刘布鸣[3] 黄艳[3] 莫建光 CHEN Qiu-hong;LIU Peng;WEI Ying-liang;LIU Bu-ming;HUANG Yan;MO Jian-guang(Guangxi Research Center of Analysis and Testing,Nanning 530022,China;Nanyang Second General Hospital,Nanyang 473012,Henan,China;Guangxi Institute of Traditional Medical and Pharmaceutical Science,Nanning 530022,China)

机构地区：[1]广西分析测试研究中心,南宁530022 [2]南阳市第二人民医院,河南南阳473012 [3]广西中医药质量标准研究重点实验室,南宁530022

出　　处：《湖北农业科学》2020年第14期156-158,共3页Hubei Agricultural Sciences

摘　　要：利用溶剂提取法对显脉金花茶(Camellia euphlebia Merr.ex Sealy)干燥叶乙酸乙酯部位提取分离,并测定其含量。结果表明,从显脉金花茶叶片中乙酸乙酯部位首次分离得到化合物山奈酚(kaempferol);建立了HPLC法对显脉金花茶叶中山奈酚的含量测定方法,山奈酚含量在0.10~0.40μg/mL范围内线性关系良好,平均回收率为99.84%(RSD=1.59%),山奈酚含量为0.3182~0.3640 mg/g。Solvent extraction method was used to extract and separate the ethyl acetate part of dried leaves of Camellia euphlebia Merr.ex Sealy,and determine its content.The results showed that the compound kaempferol was first isolated from the ethyl acetate part of Camellia euphlebia Merr.ex Sealy;the content determination method of kaempferol in Camellia euphlebia Merr.ex Sealy was established by HPLC.The kaempferol ranged from 0.10 to 0.40μg/mL.The internal linear relationship was good,the average recovery rate was 99.84%(RSD=1.59%),and the measured content of kaempferol was 0.3182~0.3640 mg/g.

关 键 词：显脉金花茶(Camellia euphlebia Merr.ex Sealy) 山奈酚 含量测定 

分 类 号：R284.1[医药卫生—中药学] R282.71[医药卫生—中医学]