2型糖尿病患者ABCA1 R219K基因多态性分布及其与血脂水平的相关性研究  

Study on Distribution of ABCA1 R219K Gene Polymorphism and Its Correlation with Blood Lipid Levels for Type 2 Diabetes Mellitus Patients

作　　者：洪雄新[1] 李观强[1] 杨辉[1] 李小峰[1] 万彦彬[1] 郭中秋[1] 杨素珍[1] HONG Xiong-xin;LI Guan-qiang;YANG Hui;LI Xiao-feng;WAN Yan-bin;GUO Zhong-qiu;YANG Su-zhen(Longgang District People’s Hospital of Shenzhen,Guangdong Shenzhen 518172,China)

机构地区：[1]深圳市龙岗区人民医院,广东深圳518172

出　　处：《现代检验医学杂志》2020年第4期14-17,共4页Journal of Modern Laboratory Medicine

摘　　要：目的研究三磷酸腺苷结合盒转运子A1(ABCA1)R219K基因多态性在2型糖尿病(T2DM)患者中的分布情况,探讨其与2型糖尿病患者血脂的关系。方法选取2015年12月~2017年4月期间深圳市龙岗区人民医院收治的85例T2DM患者和90例健康体检对照组纳入研究,对两组研究对象进行血液标本采集,采用聚合酶链反应(PCR)-限制性片段长度多态性(RFLP)检测ABCA1 R219K等位基因和基因型频率情况,对比两组患者血脂水平以及研究组不同基因型患者血脂水平情况,进一步探讨T2DM患者ABCA1 R219K基因多态性及其与血脂水平的关系。结果两组研究对象TC,TG,HDL-C和LDL-C水平对比差异均无统计学意义(t=0.191,0.853,0.755和0.597,均P>0.05)。两组研究对象ABCA1 R219K等位基因和基因型频率对比差异均无统计学意义(t=0.860,2.393,均P>0.05)。ABCA1 R219K等位基因三种基因型之间TC,TG和LDL-C水平差异无统计学意义(F=0.02,0.14,2.00和0.06,P>0.05);KK基因型HDL-C水平明显高于RR和RK基因型,差异有统计学意义(t=2.543和2.630,P<0.05);RR和RK基因型之间HDL-C水平差异无统计学意义(t=0.238,P>0.05)。结论ABCA1 R219K基因多态性并不是T2DM发病的遗传学标志,但其变异参与了T2DM患者脂代谢异常的调节。Objective To study ATP-binding cassette transporter 1(ABCA1)R219K gene polymorphism distribution and its correlation with blood lipid levels for type 2 diabetes mellitus(T2DM)patients.Methods 85 T2DM patients and 90 healthy physical examination control group were analyzed in the Longgang District People’s Hospital of Shenzhen from December 2015 to April 2017.Blood samples were collected from both groups,and ABCA1 R219K allele and genotype frequencies were detected by polymerase chain reaction-restriction fragment length polymorphism(RFLP),and investigated the relationship between ABCA1 R219K and serum lipid levels in patients with T2DM by comparing the serum lipid levels of the two groups and the patients with different genotypes in the study group.Results TC(total cholesterol),TG(triglyceride),HDL-C(high density lipid-cholesterol)and LDL-C(low density lipid-cholesterol)levels between groups showed no statistically significant difference(t=0.191,0.853,0.755 and 0.597,all P>0.05).ABCA1 R219K alleles and genotype frequency between groups showed no statistically significant difference(t=0.860,2.393,all P>0.05).TC,TG and LDL-C levels among patients with three ABCA1 R219K allele genotypes showed no statistically significant difference(F=0.02,0.14,2.00 and 0.06,all P>0.05).HDL-C levels in KK genotype patients were significantly higher than RR and RK genotype patients(t=2.543 and 2.630,P<0.05).HDL-C levels between RR patients and RK patients showed no statistically significant difference(t=0.238,P>0.05).Conclusion ABCA1 R219K gene polymorphism can’t be considered as the genetic marker of T2DM,but its gene variations are involved with patient’s abnormal lipid metabolism.

关 键 词：2型糖尿病 三磷酸腺苷结合盒转运子A1 R219K 基因多态性 血脂 

分 类 号：R587.1[医药卫生—内分泌] Q503[医药卫生—内科学]