滇重楼地上部分化学成分研究  

Studies on the chemical constituents in the aerial parts of Paris polyphylla var.Yunnanensis

作　　者：陶俊杰 杨杰[1] 闻晓东[1] TAO Junjie;YANG Jie;WEN Xiaodong(State Key Laboratory of Natural Medicines,School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198,China)

机构地区：[1]中国药科大学中药学院,天然药物活性组分与药效国家重点实验室,南京211198

出　　处：《中国药科大学学报》2020年第4期454-460,共7页Journal of China Pharmaceutical University

基　　金：国家重点研发计划资助项目(No.2019YFC1711000);国家自然科学基金资助项目(No.81773877)。

摘　　要：利用硅胶柱色谱、MCI柱色谱和制备高效液相色谱等技术对滇重楼地上部分的化学成分进行研究,结果从滇重楼地上部分90%乙醇提取物的正丁醇萃取部分中分离得到6个甾体皂苷类化合物,根据理化性质和波谱数据鉴定为26-O-β-D-glucopyranosyl-kryptogenin-3-O-α-L-rhamnopyranosyl-(1→4)-α-L-rhamnopyranosy-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside(1),dioseptemloside G(2),polyphylloside Ⅲ(3),chonglouoside SL-19(4),protodioscin(5),chonglouoside SL-5(6)。其中化合物1为新化合物,化合物2为首次从重楼属植物中分离得到。对以上化合物进行促血小板聚集活性以及细胞毒性评价,结果发现:6个化合物均未表现出明显的促血小板聚集作用;化合物2和4对于人结肠癌细胞HT29具有较强的细胞毒性。Six steroidal saponins were isolated from the n-butanol extract of 90%ethanol extract of the aerial parts of Paris polyphylla var.yunnanensis by silica gel for column chromatography,MCI column chromatography and preparative high performance liquid chromatography(HPLC).According to the physicochemical properties and spectral data,they were identified as 26-O-β-D-glucopyranosyl-kryptogenin-3-O-α-L-rhamnopyranosyl-(1→4)-α-L-rhamnopyranosy-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside(1),dioseptemloside G(2),polyphylloside Ⅲ(3),chonglouoside SL-19(4),protodioscin(5),chonglouoside SL-5(6).Among these,compound 1 was a new compound,and compound 2 was obtained from Paris plants for the first time.The platelet aggregation activities and cytotoxicities of the above compounds were evaluated.The results showed that none of the isolated compounds showed significant platelet aggregation activity;compound 2 and 4 exhibited strong cytotoxicity against human colon adenocarcinoma cell line HT29.

关 键 词：滇重楼 甾体皂苷 分离鉴定 血小板聚集 细胞毒性 

分 类 号：R931.6[医药卫生—生药学]