超声微泡辅助间充质干细胞携载姜黄素胶束对大鼠前列腺炎的作用  

The efficacy of ultrasound microbubble assisted mesenchymal stem cells carrying curcumin micelles on rat prostatitis

作　　者：买冰洁 王婵媛 井玥文 王筱冰[1] MAI Bingjie;WANG Chanyuan;JING Yuewen;WANG Xiaobing(School of Life Sciences,Shaanxi Normal University,Xi′an 710119,Shaanxi,China)

机构地区：[1]陕西师范大学生命科学学院,陕西西安710119

出　　处：《陕西师范大学学报（自然科学版）》2020年第5期78-86,共9页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金(81972900);陕西省自然科学基金(2019JZ-13)。

摘　　要：慢性细菌性前列腺炎(CBP)是一种常见的男性泌尿系统疾病,而骨髓间充质干细胞(BMSCs)具有趋向炎症组织、分泌抗炎物质的特性;因此,本研究探索了借助超声联合微泡(USM)打开血-前列腺屏障(BPB),以辅助骨髓间充质干细胞携载姜黄素胶束(BMSCs-CM)对大鼠慢性细菌性前列腺炎治疗效果的影响。实验通过密度梯度离心和表面抗原检测方法分离鉴定BMSCs,采用细胞存活与胞内姜黄素荧光定量筛选构建BMSCs-CM的最佳参数,利用荧光成像显示USM辅助BMSCs-CM在前列腺组织的靶向富集,以前列腺指数和炎症因子水平评估USM辅助BMSCs-CM对CBP的治疗效果。结果发现,质量浓度为100μg/mL的姜黄素胶束与BMSCs共同孵育4 h为构建BMSCs-CM的最佳条件;USM能显著促进BMSCs-CM在前列腺炎组织的富集,同时USM辅助BMSCs-CM处理可有效降低大鼠CBP的炎症反应。该研究结果为CBP的临床治疗提供了新思路。Chronic bacterial prostatitis(CBP)is a common male urinary disease,while bone marrow mesenchymal stem cells(BMSCs)have the characteristics of accumulation in inflammatory tissues and secreting anti-inflammatory substances.On these basis,ultrasound combined with microbubbles(USM)was used to open the blood-prostate barrier(BPB)to enhance the CBP treatment effect of BMSCs carrying curcumin micelles(BMSCs-CM).BMSCs isolation and identification were performed by density gradient centrifugation and surface antigen detection.The cell survival and intracellular curcumin fluorescence quantification were carried out to select the optimal parameters for constructing BMSCs-CM.Fluorescence imaging was used to show the targeted enrichment of USM assisted BMSCs-CM in prostate tissue.The efficacy of USM assisted BMSCs-CM on CBP was evaluated by prostate index and inflammatory factor levels.Results showed that the combined incubation of 100μg/mL CM and BMSCs for 4 h was the optimal condition for constructing BMSCs-CM;USM could significantly promote the enrichment of BMSCs-CM in prostate tissue,and USM assisted BMSCs-CM treatment could effectively reduce the inflammation of CBP.Our finding provides a new idea for the clinical treatment of CBP.

关 键 词：超声微泡 血-前列腺屏障 骨髓间充质干细胞 姜黄素 慢性细菌性前列腺炎 

分 类 号：Q28[生物学—细胞生物学]