MACF1对成骨细胞微丝骨架和细胞力学性能的调节作用  

The regulatory role of MACF1 on actin filaments and cell mechanical property of osteoblast

作　　者：胡丽芳[1,2] 吴自祥 丛晓岚 Mitchel Alioscha-Perez 王哲 蒋冬梅[2] 骞爱荣[1] Hichem Sahli[2,3,4] HU Lifang;WU Zixiang;CONG Xiaolan;ALIOSCHA-PEREZ Mitchel;WANG Zhe;JIANG Dongmei;QIAN Airong;SAHLI Hichem(School of Life Sciences,Northwestern Polytechnical University,Xi′an 710072,Shaanxi,China;School of Computer Science,Northwestern Polytechnical University,Xi′an 710072,Shaanxi,China;Department of Electronics&Informatics(ETRO),Vrije Universiteit Brussel(VUB),Brussels B-1050,Belgium;Interuniversity Microelectronics Center,Leuven 3001,Belgium)

机构地区：[1]西北工业大学生命学院,陕西西安710072 [2]西北工业大学计算机学院,陕西西安710072 [3]比利时布鲁塞尔自由大学电子信息学院,比利时布鲁塞尔B-1050 [4]比利时校际微电子中心,比利时勒芬3001

出　　处：《陕西师范大学学报（自然科学版）》2020年第5期98-104,111,共8页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金(81772017);中国博士后科学基金(2018T111099,2017M610653)。

摘　　要：为探索细胞骨架关键交联分子微管微丝交联因子1(microtubule actin crosslinking factor 1,MACF1)对成骨细胞微丝骨架和细胞力学性能的调节作用,以MACF1低表达的小鼠成骨细胞及其对照细胞为研究对象,通过细胞免疫荧光染色和激光扫描共聚焦显微镜,观察成骨细胞微丝骨架;运用微丝图像分析系统对微丝特性进行定量分析;采用原子力显微镜检测细胞弹性模量。结果发现,与对照组相比,MACF1低表达显著改变了小鼠成骨细胞的微丝分布角度,增加了成骨细胞的微丝长度与数量,显著减小了成骨细胞的刚度。研究结果为深入认识MACF1在成骨细胞中的功能奠定了实验基础,并为由成骨细胞功能改变引起的骨质疏松等骨骼疾病防治研究提供了新靶标。To investigate the regulation effects of microtubule actin crosslinking factor 1(MACF1),a critical cytoskeletal crosslinker,on the actin filaments and cell mechanical property of osteoblast,the MACF1 knockdown(MACF1-KD)osteoblast in mice and the corresponding control cell line were adopted.The actin filaments were treated with immunofluorescence staining and observed by laser scanning confocal microscope.The images of actin filaments were quantitatively analyzed by a robust actin filaments analysis framework.The cell Young′s modulus was determined by atomic force microscope.Results showed that in comparison with the control samples,knockdown of MACF1 resulted in significantly altered angle distribution of the actin filaments,increased length and number of actin filaments,and significantly decreased cell stiffness of osteoblast.These results lay an experimental foundation for further understanding of the function of MACF1 in osteoblast,and provide a new target for the prevention and treatment of bone diseases(e.g.osteoporosis)caused by the function changes of osteoblast.

关 键 词：MACF1 成骨细胞 微丝 图像分析 细胞力学性能 

分 类 号：Q28[生物学—细胞生物学]