毛乌素沙地锦鸡儿(Caragana)根系微域细菌群落多样性特征  

Diversity of bacterial communities in the rhizocompartments of Caragana in the Mu Us Desert

作　　者：周子渊 于明含 丁国栋 高广磊 何莹莹 Zhou Ziyuan;Yu Minghan;Ding Guodong;Gao Guanglei;He Yingying(Key Laboratory of State Forestry Administration on Soil and Water Conservation/MOE Engineering Research Center of Forestry Ecological Engineering/Yanchi Ecology Research Station of the Mu Us Desert,School of Soil and Water Conservation,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学水土保持学院水土保持国家林业局重点实验室/林业生态工程教育部工程研究中心/宁夏盐池毛乌素沙地生态系统国家定位观测研究站,北京100083

出　　处：《中国沙漠》2020年第4期128-137,共10页Journal of Desert Research

基　　金：国家重点研发计划课题(2016YFC0500905)。

摘　　要：基于16S rRNA高通量基因测序技术,对毛乌素沙地小叶锦鸡儿(Caragana microphylla)、柠条锦鸡儿(Caragana korshinskii)根系微域(即根系、根际土、根区土、灌丛间空白土)间的细菌群落多样性和结构差异性进行表征。本研究对各根系微域间细菌群落的Alpha多样性指数进行了单因素方差分析以及基于OTU水平的PCA分析,探究其在根系微域间Alpha和Beta多样性的层级变化,证实了有关植物根系微域生态位分化的报道,并发现锦鸡儿属植物根系微域间细菌群落的多样性和结构组成随着4个微域类型由外及内呈现出显著的层级差异性(P<0.05)。通过对优势细菌群组的结构组成分析,发现锦鸡儿属植物对特定细菌群组具有显著的向根系内筛选富集的作用(P<0.05)。这种植物通过根系微域对特定细菌群组的逐级筛选富集作用,是导致锦鸡儿属植物灌丛下不同生态位间细菌群落结构和组成发生层级变异的主要原因。Based on 16 S rRNA high-throughput genome sequencing,the bacterial diversity and structural variability in the rhizocompartments(i. e. root,rhizosphere soil,root zone soil and inter-shrub bulk soil)of two Caragana spp.(Caragana microphylla and Caragana korshinskii) in the Mu Us Desert are studied. In this study,one-way ANOVA and PCA analysis based on OTU level with Euclidean distance were performed on the Alpha diversity indeices and Beta diversity of bacterial communities in each rhizocompartments,respectively.Then we confirmed the reports on the niche differentiation of plant rhizocompartments,the diversity and structural composition of the bacterial community in the rhizocompartments of desert leguminous plants showed significant differences,with a hierarchical transition of the four rhizocompartment types from outside to inside. We found that desert leguminous plants showed significant hierarchical filtration and enrichment of soil microbial communities under shrubs(P<0. 05). The stratified selection and enrichment of specific bacterial groups by the rhizocompartments of plant is one of the main reasons for the hierarchical variation of bacterial community structure and composition in different ecological niches under the shrub of Caragana.

关 键 词：荒漠 锦鸡儿(Caragana) 土壤细菌 多样性 结构变异性 

分 类 号：Q938.1[生物学—微生物学]