PEG模拟干旱胁迫对白菜型春油菜芽期生长特性的影响  

Effects of PEG Simulation Drought Stress on Growth Characteristics of Spring Rapeseed(Brassica campestris L.)at Bud Stage

作　　者：高雪 尼玛扎西 刘国一 谭海运 GAO Xue;Nimazhaxi;LIU Guoyi;TAN Haiyun(State Key Laboratory of Hullless Barley and Yark Germplasm Resources and Genetic Improvement/Institute of Agricultural Resources and Environment,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa 850000,China)

机构地区：[1]省部共建青稞和牦牛种质资源与遗传改良国家重点实验室/西藏自治区农牧科学院农业资源与环境研究所,西藏拉萨850000

出　　处：《广东农业科学》2020年第7期9-17,共9页Guangdong Agricultural Sciences

基　　金：西藏自治区重点研发与转化计划(XZ-2019-NK-NS-005);西藏自治区重大科技专项(XZ201901NA05)。

摘　　要：【目的】采用不同浓度聚乙二醇6000(PEG6000)溶液模拟干旱胁迫,研究白菜型春油菜芽期幼苗根系形态特征及生理生化特性,评价不同白菜型春油菜品种(系)抗旱性强弱。【方法】设置不同浓度(0%、5%、10%、15%、20%、25%)PEG6000溶液,观察模拟干旱胁迫下4个白菜型春油菜品种(系)芽期幼苗的生长情况及生理生化特性,同时筛选抗旱性白菜型春油菜品种(系)。【结果】PEG6000溶液对4个品种(系)白菜型春油菜芽期幼苗成苗率、主根长、苗高、单株鲜重、相对活力指数等指标具有显著抑制效应。溶液浓度为15%~20%时,4个品种(系)白菜型春油菜相对活力指数在0.50~0.60之间;浓度为0%~15%时,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性与溶液浓度呈正相关关系;浓度为15%~20%时酶活性出现峰值,超过此浓度范围酶活性下降。浓度为5%~15%时,可溶性糖(SS)、可溶性蛋白(SPr)、脯氨酸(Pro)、丙二醛(MDA)含量与溶液浓度呈正相关关系。【结论】白菜型春油菜幼苗相对活力指数及苗高可作为抗旱性筛选指标,15%~20%PEG6000溶液浓度可作为白菜型春油菜模拟干旱胁迫最适浓度,筛选出抗旱性品系104005。【Objective】The polyethylene glycol 6000(PEG6000)solution was used to simulate drought stress to study the root morphological characteristics,physiological and biochemical characteristics of the seedlings of spring rapeseed(Brassica campestris L.)at bud stage,and evaluate the drought resistance of different spring rapeseed cultivars(lines).【Method】Different concentrations(0%,5%,10%,15%,20%,25%)of PEG6000 solutions were used to the study the effect of drought stress on the growth,physiological and biochemical characteristics of four spring cultivates(lines)rapeseed seedlings.Meanwhile,the drought resistant spring rapeseed cultivars(lines)were screened out.【Result】PEG6000 solutions had significant inhibitory effects on the seedling rate,main root length,seedling height,fresh quality per plant and relative vigor index of four spring rapeseed cultivars(lines)at bud stage.When the PEG6000 solution concentration was in the range of 15%-20%,the relative vigor index of spring rapeseed of four cultivars(lines)was 0.50-0.60.When the PEG6000 concentration was 0%-15%,the activity of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)were positively correlated with the stress mass fraction.The enzyme activity reached the peak when the PEG6000 concentration was 15%-20%,and then decreased.In the low stress concentration range(5%-15%),soluble sugar(SS),soluble protein(SPr),proline(Pro),malondialdehyde(MDA)were positively correlated with PEG6000 solution concentration.【Conclusion】The relative vigor index and seedling height of spring rapeseed could be used as drought resistance screening indicators,and 15%-20%PEG6000 solution concentration as the optimum concentration to simulate drought stress for spring rapeseed.And the drought resistance line 104005 was selected.

关 键 词：聚乙二醇6000 白菜型春油菜 干旱胁迫 生长特性 生理生化 抗旱性 

分 类 号：S564.4[农业科学—作物学]