化学发光法和酶联免疫法检测乙肝病毒血清的效果  

Detection of Hepatitis B Virus Serum by Chemiluminescence Assay and Enzyme-linked Immunosorbent Assay

作　　者：陈剑武 Chen Jianwu(Dongguan Kangyi Hospital,Dongguan 523000)

机构地区：[1]东莞康怡医院,东莞523000

出　　处：《数理医药学杂志》2020年第9期1277-1278,共2页Journal of Mathematical Medicine

摘　　要：目的:探讨化学发光法和酶联免疫法检测乙肝病毒血清的效果。方法:对2018年6月~2018年12月某院413例乙肝病毒筛查病人进行研究分析,采集血液标本,平均分为两份,分别采用化学发光法和酶联免疫法检测乙肝病毒血清,比较检测结果。结果:化学发光法检出乙肝病毒血清阳性189例(45.76%),酶联免疫法检出阳性125例(30.27%),差异具有统计学意义(P<0.05);酶联免疫法的中浓度HBsAg含量的批内重复率、批间重复率分别为9.48%、14.92%,显著高于化学发光法1.16%、5.47%;低浓度HBsAg含量的批内重复率、批间重复率分别为12.85%、19.47%,显著高于化学发光法2.42%、7.29%,差异具有统计学意义(P<0.05)。两种检测方法高浓度HBsAg含量的批内重复率与批间重复率比较,不具有统计学意义(P>0.05)。结论:临床检测乙肝病毒血清,应用化学发光法的准确率比酶联免疫法更高,值得推广。Objective:To explore the effect of chemiluminescent assay and enzyme-linked immunosorbent assay(Elisa)in detecting hepatitis B virus(HBV)serum.Methods:413 cases of patients with hepatitis B virus screening in a hospital from June 2018 to December 2018 were studied and analyzed.Blood samples were collected and divided into 2 samples.Two methods were used to detect hepatitis B virus serum:chemiluminescence assay and enzyme-linked immunosorbent assay(Elisa).The results were compared.Results:189 cases(45.76%)were positive by chemiluminescence assay and 125 cases(30.27%)were positive by enzyme-linked immunosorbent assay(P<0.05).The intra-assay repetition rate and inter-assay repetition rate of medium concentration HBsAg content in Elisa was 9.48%and 14.92%respectively,which were significantly higher than those of chemiluminescent method(1.16%,5.47%).The intra-assay repetition rate and inter-assay repetition rate of low concentration HBsAg content was 12.85%and 19.47%respectively,which were significantly higher than those of chemiluminescent method(2.42%,7.29%).The difference between the two methods was statistically significant(P<0.05).There was no significant difference in intra-assay repetition rate and inter-assay repetition rate of high-concentration HBsAg content between the two methods(P>0.05).Conclusion:The application of chemiluminescence method in the clinical detection of hepatitis B virus serumis is more accurate than enzyme-linked immunoassay,which is worthy of promotion.

关 键 词：乙肝病毒血清 化学发光法 酶联免疫法 应用效果 

分 类 号：R446.1[医药卫生—诊断学]