MCA在IFN-α和TNF-β基因多态性检测中的临床应用  

Melting Curve Analysis in Detection of Interferon-αand Tumor Necrosis Factor-βGenetic Polymorphism

作　　者：石彩歌[1] 肖奇志[2] 黄士荷 张素粉 王群慧[1] 陈兴[2] 聂继跃[1] 郭天棋[1] SHI Caige;XIAO Qizhi;HUANG Shihe;ZHANG Sufen;WANG Qunhui;CHEN Xing;NIE Jiyue;GUO Tianqi(Department of Obsterics,Maternal and Child Health Cares Hospital of Zhuhai,Guangdong Province,Zhuhai Guangdong 519001,China;Department of Laboratory,Maternal and Child Health Cares Hospital of Zhuhai,Guangdong Province,Zhuhai Guangdong 519001,China)

机构地区：[1]广东省珠海市妇幼保健院妇科,广东珠海519001 [2]广东省珠海市妇幼保健院检验科,广东珠海519001

出　　处：《中国卫生标准管理》2020年第16期103-107,共5页China Health Standard Management

基　　金：广东省珠海市科技计划医疗卫生项目(20181117E030067)。

摘　　要：目的建立一个基于荧光探针熔解曲线技术(MCA)的细胞因子基因多态性检测体系并验证其临床应用效果。方法以干扰素-α(IFN-α)的rs6475526位点和肿瘤坏死因子-β(TNF-β)的rs2239704、rs909253位点为检测对象,建立能够单管同步检测9种基因多态性的荧光探针熔解曲线检测体系。收集珠海市妇幼保健院2018年1月—2019年6月共计282份宫颈拭子样本开展临床试验。结果荧光探针熔解曲线基因多态性检测体系与金标准Sanger测序技术的符合率为100%。在本次临床试验中rs6475526位点主要基因型为T/C;rs2239704位点主要基因型为T/T;rs909253位点主要基因型为C/T。各位点的基因型在健康人群和患病人群中的分布具有统计学差异(P<0.05)。结论荧光探针熔解曲线体系具备准确、快速和简便的优点,适合用于细胞因子基因多态性的临床检测。Objective To develop and evaluate the detection system for cytokines polymorphism based on fluorescent probe melting curve analysis.Methods The genetic polymorphism detection system based on fluorescent probe melting curve analysis was developed for rs6475526 of interferon-α and rs2239704,rs909253 of tumor necrosis factor-β,which could identify 9 genotypes of polymorphisms in a single reaction.282 cervical swab samples(2018.1-2019.6)from Zhuhai Maternity and Child Health Hospital were chosen for clinical trials.Results The coincidence rate of melting curve analysis system with sanger sequencing was 100%.In this study,T/C was the most common genotype for rs6475526;T/T was the most common genotype for rs2239704;C/T was the most common genotype for rs909253.The genotype distribution frequency between health population and illness population was significantly different(P<0.05).Conclusion Melting curve analysis system is proved to be accurate,fast,convenient,and suitable for clinical detection of cytokinegenetic polymorphism.

关 键 词：干扰素-Α 肿瘤坏死因子-Β 基因多态性 熔解曲线分析 宫颈癌 

分 类 号：R711[医药卫生—妇产科学]