芍倍注射液对痔急性发作模型大鼠直肠肛管组织病理形态学、炎症反应的影响研究  

Effect of Shaobei injection on rectoanal pathological morphology and inflammatory response in treating acute hemorrhoids model rats

作　　者：黄丹丹[1] 刘智敏[1] 赵严冬 呙耀宇 任东林[1] Huang Dandan;Liu Zhimin;Zhao Yandong;Guo Yaoyu;Ren Donglin(Department of Anorectal Surgery,The Sixth Affiliated Hospital of Sun Yat-sen University,Guangzhou 510655,Guangdong,Chi-na;Department of Pathology,The Sixth Affiliated Hospital of Sun Yat-sen University,Guangzhou 510655,Guangdong,China)

机构地区：[1]中山大学附属第六医院肛肠外科,广东广州510655 [2]中山大学附属第六医院病理科,广东广州510655

出　　处：《结直肠肛门外科》2020年第4期451-455,共5页Journal of Colorectal & Anal Surgery

摘　　要：目的探讨芍倍注射液对痔急性发作模型大鼠直肠肛管组织病理形态学、炎症反应的影响。方法将75只大鼠随机分为对照组、模型组及治疗组,每组25只。对照组不予处理,模型组与治疗组利用6%巴豆油制备痔急性发作大鼠模型,造模成功后,模型组给予肛管注射生理盐水,治疗组给予肛管注射芍倍注射液稀释液。给药后第1天、第3天、第7天后分别比较各组大鼠直肠肛管组织病理形态学变化,给药后第7天比较各组直肠肛管系数、直肠肛管湿干重差、直肠毛细血管通透性、细胞炎症因子水平。结果模型组大鼠的直肠肛管组织充血水肿明显,分泌物明显增加,镜下可见黏膜上皮脱落坏死,黏膜下层充血出血,血管扩张明显,伴大量急性炎症细胞浸润,直肠肛管组织病变明显,提示造模成功。治疗组直肠肛管组织充血肿胀程度和病变程度明显减轻,分泌物减少,给药后炎症细胞浸润、黏膜下充血出血明显减轻,给药后第3天,黏膜上皮开始修复。治疗组给药后各时点的炎症细胞浸润评分、充血/出血评分均低于模型组(均P<0.05)。给药后第3天、第7天,治疗组的血管扩张评分、上皮细胞坏死/损伤评分均低于模型组(均P<0.05)。模型组直肠肛管系数、直肠肛管湿干重差较对照组大,血清中的TNF-α、IL-1、IL-6水平及伊文思蓝含量较对照组均增高(均P<0.05);治疗组直肠肛管系数、直肠肛管湿干重差较模型组小,血清中的TNF-α、IL-1、IL-6水平及伊文思蓝含量较模型组均下降(均P<0.05)。结论芍倍注射液能有效改善痔急性发作模型大鼠直肠肛管病理组织形态,抑制局部炎症反应,促进直肠肛管黏膜修复。Objectives To investigate the effect of Shaobei injection on the rectoanal pathological morphology and inflammatory response in treating acute hemorrhoids model rats.Methods Seventy-five rats were randomly divided into control group,model group,and treatment group,with 25 rats in each group.The control group received no intervention.In the model group and treat⁃ment group,an acute hemorrhoid model was induced by 6%croton oil.After the establishment of experimental model,the model group was treated with injection of normal saline into the anus,and the treatment group was treated with injection of Shaobei so⁃lution into the anus.Rectoanal pathological morphology changes were compared at 1,3,and 7 days after the intervention.Recto⁃anal coefficient,difference between wet and dry weight of the rectum and anus,capillary permeability,and cytokine levels were compared at 7 days after the intervention.Results Rats in the model group had significantly increased hyperemia,edema,and secretion.Microscopic examination showed epithelial exfoliation and necrosis,submucosal hyperemia,hemorrhage,vascular dila⁃tion,and infiltration of acute inflammatory cells,the rectoanal tissue is obviously diseased,indicating that the model was success⁃fully established.In contrast,rats in the treatment group had less hyperemia,edema,lesions,and secretion.After the injection of Shaobei solution,the infiltration of inflammatory cells and submucosal hyperemia,hemorrhage were alleviated.The mucosal epithe⁃lium repair was noticeable at 3 days after treatment.Scores of infiltration of inflammatory cells and hyperemia/hemorrhage at each time point were significantly lower in the treatment group than in the model group(P<0.05).Scores of vascular dilation and epithelial cells necrosis/lesion at 3 and 7 days after treatment were also significantly lower in the treatment group(P<0.05).Compared with the control group,the model group had a significantly higher rectoanal coefficient,difference between the wet and dry weight of the rectum and anus

关 键 词：痔急性发作 芍倍注射液 病理形态学 炎症反应 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]