水椰八角铁甲不同日龄和性别成虫表皮碳氢化合物的种类及动态  

Categories and dynamics of cuticular hydrocarbons in Octodonta nipae adults with different ages and sexes

作　　者：蒲宇辰 向海军 黄斌[1,2] 侯有明 PU Yu-Chen;XIANG Hai-Jun;HUANG Bin;HOU You-Ming(State Key Laboratory of Ecological Pest Control for Fujian-Taiwan Crops,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian Provincial Key Laboratory of Insect Ecology,College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,China;School of Biological Science and Biotechnology,Minnan Normal University,Zhangzhou 363000,Fujian Province,China;Fapon Biotech Inc.,Dongguan 523808,Guangdong Province,China)

机构地区：[1]福建农林大学闽台作物有害生物生态防控国家重点实验室,福州350002 [2]福建农林大学植物保护学院福建省昆虫生态学重点实验室,福州350002 [3]闽南师范大学生物科学与技术学院,福建漳州363000 [4]菲鹏生物股份有限公司,广东东莞523808

出　　处：《环境昆虫学报》2020年第4期838-846,共9页Journal of Environmental Entomology

基　　金：国家重点研发计划课题(2017YFC1200605);福建省科技重大专项(2017NZ0003-1-6)。

摘　　要：水椰八角铁甲Octodonta nipae(Maulik)是一种严重为害棕榈科植物的外来入侵害虫。为了明确日龄和性别等个体因素对水椰八角铁甲成虫表皮碳氢化合物的影响,本研究采用化学萃取法和气相色谱-质谱联用技术,分析了不同日龄的水椰八角铁甲雌、雄成虫表皮碳氢化合物的组成和含量。结果表明,水椰八角铁甲成虫的表皮碳氢化合物由21种C 18-C 30的碳氢化合物构成,包括11种正构烷烃、2种单甲基烷烃、1种多甲基烷烃、3种正构烯烃、1种单甲基烯烃和3种环烷烃。不同日龄和性别成虫的表皮碳氢化合物的组成种类相同,但是一些组分的含量有明显变化,例如1 d、5 d、10 d和15 d雌、雄成虫之间正二十四烷、正二十六烷、2-甲基十九烷和1-二十烯的含量存在显著差异。水椰八角铁甲成虫表皮碳氢化合物具有性二型,如性成熟雄虫的表皮的正二十烷、正二十一烷、正二十四烷、正二十六烷、2-甲基十九烷、10-甲基二十烷、1-二十烯和1-二十二烯的含量显著高于性成熟雌虫。本文的研究结果可为检验检疫过程中水椰八角铁甲近缘种,以及日龄、性别和性成熟的快速鉴定提供支持。The nipa palm hispid Octodonta nipae(Maulik)is a species of invasive pests,which can cause serious damage to palms.To clarify the effects of age and sex on cuticular hydrocarbons(CHCs)in O.nipae adults,the components and contents of CHCs from O.nipae male and female adults at different ages were analyzed by chemical extraction and gas chromatography-mass spectrometry.The results indicated some differences in the content of some CHCs components from O.nipae adults of different age and sex,but the CHCs profiles showed similarities.Total of 21 hydrocarbons,ranging from C 18 to C 30,were separated from the epidermis of O.nipae adults,including 11 n-alkanes,2 monomethyl alkanes,1 polymethyl alkane,3 n-alkenes,1 monomethyl alkene and 3 cycloalkanes.There were significant differences in the content of n-C 24,n-C 26,2-meC 19 ane,and 1-C 20 ene,among O.nipae male or female adults at 1 d,5 d,10 d and 15 d after eclosion.CHCs of O.nipae adults presented sexual dimorphism.The content of some chemicals,containing n-C 20,n-C 21,n-C 24,n-C 26,2-meC 19 ane,10-meC 20 ane,1-C 20 ene,and 1-C 22 ene,from the mature male adults were significantly higher than those of mature females.Our findings provided a support for the quick identification of closely related species,age,sex and sexual maturity of O.nipae during border inspection and quarantine.

关 键 词：水椰八角铁甲 表皮碳氢化合物 化学信息素 性别二态性 

分 类 号：Q968.1[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治]