甲基硬脂酸酯和E-β-罗勒烯对中华蜜蜂育王质量的影响  

Effects of methyl stearate and E-beta-ocimene on the reared queen quality of the Chinese honeybee,Apis cerana cerana(Hymenoptera:Apidae)

作　　者：胡景华[1,2] 赵方媛 张丽珍 江武军[2] 廖春华 颜伟玉[1] HU Jing-Hua;ZHAO Fang-Yuan;ZHANG Li-Zhen;JIANG Wu-Jun;LIAO Chun-Hua;YAN Wei-Yu(Honeybee Research Institute of Jiangxi Agricultural University,Nanchang 330045,China;Apiculture Research Institute of Jiangxi Province,Nanchang 330052,China)

机构地区：[1]江西农业大学蜜蜂研究所,南昌330045 [2]江西省养蜂研究所,南昌330052

出　　处：《环境昆虫学报》2020年第4期973-978,共6页Journal of Environmental Entomology

基　　金：国家自然科学基金(31460641,31660696);江西省现代农业产业技术体系建设专项资金(JXARS-14)。

摘　　要：为研究育王过程中添加蜜蜂幼虫信息素成分甲基硬脂酸酯和E-β-罗勒烯对中华蜜蜂育王质量的影响,本试验结合免移虫育王技术,在幼虫60~64 h时向王台内注入1μL配置好的信息素(浓度梯度分别为0%、0.1%、1.0%、10.0%),待蜂王出房后测定蜂王个体指标及蜂王卵巢Vg、hex70b和hex110基因的表达水平。与空白对照相比,添加10.0%浓度的E-β-罗勒烯组蜂王的初生重和单侧卵巢管数显著增加,同时Vg、hex70b和hex110基因表达水平也显著上升;添加1.0%的E-β-罗勒烯蜂王单侧卵巢管数量显著增加,Vg和hex110基因的表达量也显著上升;添加甲基硬脂酸酯对培育蜂王的初生重、胸部指标和卵巢相关基因表达均无显著影响,但1.0%和10.0%的甲基硬脂酸酯使蜂王卵巢管数量显著减少。结果表明在中蜂育王过程中添加E-β-罗勒烯可以在一定程度上提高蜂王的质量。In order to explore the effects of the components of brood pheromone,methyl stearate(MS)and E-beta-ocimene(eβ),on the reared queen quality of Apis cerana cerana,1μL of compound with the concentration gradients of 0%,0.1%,1.0%and 10.0%was added into queen cells respectively using a micro sampling syringe injector when queen larvae was 60~64 h old.Newly emerged queens were sampled and analyzed for the index of queens and the expression level of Vg,hex70b and hex110 genes in queen ovaries.Compares with the blank control group,10.0%treatment of eβsignificantly increased the newly-emerged weight and the ovarioles number in one ovary,as well as increased the expression level of Vg,hex70b and hex110 genes significantly in queen ovaries.In 1.0%treatment of eβ,the ovarioles number in one ovary and the expression of Vg and hex110 of queen were all increased significantly.Adding MS has no obvious effect on the newly-emerged weight,the ovarioles number and the expression level of the genes.But in 1.0%and 10.0%treatment of MS,the number of ovarioles decreased notably.The results showed that eβadded in queen-rearing process can improve the queen quality to a certain extent.

关 键 词：中华蜜蜂 甲基硬脂酸酯 E-β-罗勒烯 育王质量 

分 类 号：Q968.1[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治]