垂体瘤转化基因1(PTTG1)在肝细胞癌组织高表达且与预后不良相关  

Pituitary tumor transforming gene 1 (PTTG1) is highly expressed and associated with poor prognosis in patients with hepatocellular carcinoma

作　　者：陶恒[1,2] 杨月[3] 陈洁[4] 陆进[1,2] TAO Heng;YANG Yue;CHEN Jie;LU Jin(Department of Human Anatomy,Bengbu Medical College,Bengbu 233030,China;Key Laboratory of Transplantation of Anhui Province,Bengbu Medical College,Bengbu 233030,China;Department of Otolaryngology,First Affiliated Hospital,Bengbu Medical College,Bengbu 233030,China;Department of Diagnostics,Bengbu Medical College,Bengbu 233030,China)

机构地区：[1]蚌埠医学院人体解剖学教研室,安徽蚌埠233030 [2]蚌埠医学院组织移植安徽省重点实验室,安徽蚌埠233030 [3]蚌埠医学院第一附属医院耳鼻喉科,安徽蚌埠233030 [4]蚌埠医学院诊断学教研室,安徽蚌埠233030

出　　处：《细胞与分子免疫学杂志》2020年第4期337-343,共7页Chinese Journal of Cellular and Molecular Immunology

基　　金：安徽省教育厅自然科学研究重点项目(KJ2019A0338)。

摘　　要：目的分析垂体瘤转化基因1(PTTG1)在肝细胞癌(HCC)中的表达及其临床预后意义。方法首先利用肿瘤生存(Tumorsurvival)和肝细胞癌整合分子数据库(HCCDB)分析HCC组织PTTG1及其共表达基因的表达情况;再利用cBioPortal、MetaScape和Kaplan-Meier Plotter数据库分析PTTG1基因在HCC中共表达基因的突变、基因本体(GO)功能和京都基因与基因组百科全书(KEGG)富集情况,并分析共表达基因对HCC患者总体生存(OS)的影响;最后利用肿瘤免疫评估资源库(TIMER)数据库分析PTTG1基因在HCC免疫微环境中的表达及与免疫细胞的关系,并分析各种免疫细胞对HCC患者OS的影响。结果PTTG1基因在HCC组织中高表达,且不利于HCC患者的OS;PTTG1基因在HCC中有19个显著共表达基因,并且其共表达基因在HCC中也高表达,同样不利于HCC患者的OS;PTTG1基因及其共表达基因的GO功能主要富集在细胞分裂、有丝核分裂和蛋白激酶结合等功能上,而KEGG富集在细胞循环、p53信号通路和人类嗜T细胞病毒(HTLV)感染等通路上;PTTG1及其共表达基因在HCC中均发生一定的突变,并显著缩短HCC患者的OS和DFS;PTTG1基因在HCC免疫微环境中与各种免疫细胞的表达正相关,但各种免疫细胞的表达同样也不利于HCC患者的OS。结论PTTG1高表达不利于HCC患者的生存预后。Objective To analyze the expression of pituitary tumor transforming gene 1(PTTG1)in hepatocellular carcinoma(HCC)and its clinical prognostic implication.Methods Firstly,the Tumorsurvival and HCCDB databases were used to analyze the expression of PTTG1 gene and its co-expressed genes in HCC tissues.Secondly,cBioPortal,MetaScape,Kaplan-Meier Plotter databases were used to analyze the mutations,GO functions and KEGG of the co-expressed genes of PTTG1 in HCC,and to analyze the effects of co-expressed genes on the overall survival(OS)and prognosis of HCC patients.Finally,the TIMER database was used to analyze the expression of PTTG1 gene in the HCC immune microenvironment and its relationship with immune cells,and the impact of various immune cells on OS and prognosis of HCC patients.Results PTTG1 gene was highly expressed in HCC tissues and was not conducive to OS of HCC patients.PTTG1 had 19 significant co-expressed genes in HCC,and its co-expressed genes were also highly expressed in HCC,which was also not conducive to OS of HCC patients.The GO function of PTTG1 gene and its co-expressed genes was mainly enriched in cell division,mitosis and protein kinase binding,while KEGG was enriched in cell cycle,p53 signaling pathway and human T-lymphotropic virus(HTLV)infection.PTTG1 and its co-expressed genes were mutated to some extent in HCC,and significantly shortened OS and DFS in HCC patients.PTTG1 gene was positively correlated with the expression of various immune cells in the immune microenvironment of HCC,but the expression of various immune cells was also not conducive to OS of HCC patients.Conclusion High expression of PTTG1 is not conducive to survival and prognosis of HCC patients.

关 键 词：垂体瘤转化基因1(PTTG1) 总体生存 肝细胞癌 

分 类 号：Q811.4[生物学—生物工程] R392.1[医药卫生—免疫学]