干酪乳杆菌ATCC334抑制胶原蛋白诱导关节炎(CIA)大鼠炎性因子的分泌及关节病变  

Lactobacillus casei ATCC334 inhibits the secretion of inflammatory factors and joint lesions in collagen-induced arthritis (CIA) rats

作　　者：李鑫 吴刚 金向楠 任立冰 沈晖[3] LI Xin;WU Gang;JIN Xiangnan;REN Libing;SHEN Hui(Department of Rheumatology,First Affiliated Hospital,Jinzhou Medical University,Jinzhou 121001;Department of Breast Surgery,First Affiliated Hospital,Jinzhou Medical University,Jinzhou 121001;Department of Rheumatology,First Affiliated Hospital of China Medical University,Shenyang 110000,China)

机构地区：[1]锦州医科大学附属第一医院风湿免疫科,辽宁锦州121001 [2]锦州医科大学附属第一医院乳腺外科,辽宁锦州121001 [3]中国医科大学附属第一医院风湿免疫科,辽宁沈阳110000

出　　处：《细胞与分子免疫学杂志》2020年第6期487-491,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81373219);辽宁省教育厅基金(jc2019009)。

摘　　要：目的研究干酪乳杆菌ATCC334对胶原蛋白诱导关节炎(CIA)大鼠炎性因子分泌及关节破坏的影响并探讨其可能机制。方法雌性Wistar大鼠随机分为正常对照组、CIA组、ATCC334组,每组7只。CIA组和ATCC334组大鼠尾根部皮内注射牛2型胶原蛋白建立CIA模型,对照组和CIA组大鼠每日500μL生理盐水灌胃,ATCC334组大鼠造模同时每日500μL干酪乳杆菌ATCC334溶液[2×10^8集落形成单位(CFU)/mL]灌胃,连续28 d。采用关节炎指数及后足爪增长容积评价关节炎严重程度、HE染色观察关节病变情况,ELISA检测大鼠血清中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、IL-17水平。结果与CIA组相比,ATCC334组关节炎指数、后足爪增长容积显著降低,关节病变明显减轻,血清TNF-α、IL-6、IL-17水平明显降低。结论干酪乳杆菌ATCC334能够显著降低CIA大鼠炎性因子的分泌,减轻关节骨质破坏。Objective To study the effects of Lactobacillus casei ATCC334 on the secretion of inflammatory factors and joint destruction in collagen-induced arthritis(CIA)rats and explore the possible mechanism.Methods Female Wistar rats were randomly divided into normal control group,CIA group and ATCC334 group with 7 rats each.The CIA model was established by intradermal injection of bovine type 2 collagen into the tail root of rats in the CIA group and ATCC334 group.The rats in the control group and CIA group were intragastrically perfused with 500μL normal saline every day,and simultaneously the rats in the ATCC334 group were intragastrically perfused with Lactobacillus casei ATCC334 solution[2×10^8 colony forming unit(CFU)/mL]for continuous 28 days.Arthritis index and increased hind paw volume were used to evaluate the severity of arthritis.HE staining was used to observe the joint lesions.Levels of tumor necrosis factorα(TNF-α),interleukin-6(IL-6)and IL-17 in the serum of rats were detected by ELISA.Results Compared with the CIA group,the indices of arthritis index,increased posterior paw volume,joint lesions and TNF-α,IL-6 and IL-17 levels were significantly reduced in the serum of the ATCC334 group.Conclusion Lactobacillus casei ATCC334 can significantly reduce the secretion of inflammatory factors and joint bone damage in CIA rats.

关 键 词：胶原蛋白诱导关节炎(CIA) 干酪乳杆菌 ATCC334 肿瘤坏死因子α(TNF-α) 白细胞介素6(IL-6) 白细胞介素17(IL-17) 

分 类 号：R593.22[医药卫生—内科学] R392.12[医药卫生—临床医学]