山地五月茶提取物对乳腺癌细胞MDA-MB-231增殖的抑制活性研究  

Inhibitory Activity of Extracts of Antidesma montanum on the Proliferation of Breast Cancer Cells MDA-MB-231

作　　者：马伏宁[1] 黄东梅[1] 宋顺[1] 吴斌[1] 郭刚[1] 王达新[1] 谭琳[1] MA Funing;HUANG Dongmei;SONG Shun;WU Bin;GUO Gang;WANG Daxin;TAN Lin(Haikou Experimental Station,Chinese Academy of Tropical Agricultural Sciences/Hainan Key Laboratory of Banana Genetic Improvement,Haikou,Hainan 571101,China)

机构地区：[1]中国热带农业科学院海口实验站/海南省香蕉遗传改良重点实验室,海南海口571101

出　　处：《热带作物学报》2020年第8期1693-1699,共7页Chinese Journal of Tropical Crops

基　　金：中国热带农业科学院基本科研业务费专项(No.1630092019002)。

摘　　要：为深入了解海南岛热带野生植物的保健功能及其活性成分,用CCK-8法检测山地五月茶(Antidesma montanum)果和叶的乙醇提取物对MDA-MB-231乳腺癌细胞增殖的抑制活性。结果表明,山地五月茶果和叶的乙醇提取物作用72 h对细胞的半数抑制浓度(IC50)分别为483μg/mL和306μg/mL。叶的乙醇提取物分别经石油醚、乙酸乙酯、正丁醇萃取,比较各有机溶剂萃取相及剩余水相对乳腺癌细胞抑制增殖活性,结果显示乙酸乙酯相表现出较高的活性,其处理细胞72 h的IC50为150.5μg/mL。划痕实验和Transwell检测结果表明,乙酸乙酯相提取物具有抑制MDA-MB-231细胞迁移和侵袭的能力。流式细胞仪检测结果表明,乙酸乙酯相提取物能够使MDA-MB-231细胞周期S期显著延长。UPLC-MS/MS分析结果显示,乙酸乙酯相含有穗花杉双黄酮活性成分。穗花杉双黄酮处理MDA-MB-231细胞72 h的抑制增殖IC50为192.6μg/mL,表明其是山地五月茶抑癌活性成分之一。In order to understand the health function and active components of tropical wild plants in Hainan Island the ethanol extract of the fruits and leaves of Antidesma montanum inhibiting the proliferation of breast cancer cells MDA-MB-231 by the CCK-8 method was studied in this research.The IC50 were 483μg/mL and 306μg/mL after 72 h treatment.The ethanol extract of the leaves of A.montanum was separated by petroleum ether,ethyl acetate,n-butanol and water respect.The ethyl acetate phase showed higher inhibitory activity than other phases,and the inhibition proliferation IC50 was 150.5μg/mL after 72 h treatment.Through the wound healing and transwell test,the ethyl acetate phase showed inhibition in migration and invasion activity of MDA-MB-231 cells.The active components could make the S phase in cell division delayed by flow cytometry detection.One of the components was identified as amentoflavone analyzed by UPLC-MS/MS.The IC50 of amentoflavone inhibition proliferation of MDA-MB-231 cells was 192.6μg/mL after 72 h treatment,which showed it’s one of the active ingredients in the leaves of A.montanum.

关 键 词：天然产物 活性成分 乳腺癌 山地五月茶 

分 类 号：R284.2[医药卫生—中药学]