黏性红酵母WP3产培养基条件的响应面优化  

Optimization of Medium Conditions Produced by Rhodotorula mucilaginosa WP3 with Response Surface Methodology

作　　者：王蓉 WANG Rong(Library of Hunan Polytechnic of Environment and Biology,Hengyang 421005 China)

机构地区：[1]湖南环境生物职业技术学院图书馆,湖南衡阳421005

出　　处：《科技创新与生产力》2020年第9期81-85,共5页Sci-tech Innovation and Productivity

基　　金：湖南环境生物职业技术学院2019年青年基金项目(ZK2019-04)。

摘　　要：应用苹果皮上分离的黏性红酵母(Rhodotorula mucilaginosa)WP3进行类胡萝卜素的合成。首先进行单因素试验,接着进行方差分析得到显著性因素:果糖质量浓度、酵母粉质量浓度、初始pH值;然后应用响应面法进一步优化类胡萝卜素生产,得到回归模型和最优工艺参数;最后以最优参数发酵得到的实际类胡萝卜素产量与预测值比较,证实所得回归模型可信度高。Synthesis of Carotenoid by Rhodotorula mucilaginosa WP3 isolated from apple peel was studied.First of all,single factor test was carried out,and then variance analysis was carried out to obtain significant factors:fructose mass concentration,yeast mass concentration,initial pH value.Then,response surface methodology(RSM)was applied to optimize the production of Carotenoid,and the regression model and the optimal process parameters were obtained.Finally,the actual Carotenoid yield obtained by the optimal fermentation parameters was compared with the predicted value,which confirmed that the regression model was reliable.

关 键 词：黏性红酵母 类胡萝卜素 单因素试验 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]