IL-1β及TNF-α促进大鼠肠平滑肌细胞表达胶质细胞源性神经营养因子(GDNF)并抑制其受体表达  

IL-1β and TNF-α up-regulate the expression of glial cell line-derived neurotrophic factor (GDNF) and down-regulate its receptors in rat intestinal smooth muscle cells

作　　者：陈慧玲[1] 韩悌云 高丽萍 熊彬[4] 李小丽[3] 齐国卿[3] 曾鹏云[1] 柴晔[1] 张德奎[2,3] CHEN Huiling;HAN Tiyun;GAO Liping;XIONG Bin;LI Xiaoli;QI Guoqing;ZENG Pengyun;CHAI Ye;ZHANG Dekui(Department of Hematology,Second Hospital,Lanzhou University,Lanzhou 730030,China;Department of Digestive Laboratory,Second Hospital,Lanzhou University,Lanzhou 730030,China;Department of Digestive Diseases,Second Hospital,Lanzhou University,Lanzhou 730030,China;Department of Medical Oncology,Second Hospital,Lanzhou University,Lanzhou 730030,China)

机构地区：[1]兰州大学第二医院血液科,甘肃兰州730030 [2]兰州大学第二医院消化疾病重点实验室,甘肃兰州730030 [3]兰州大学第二医院消化科,甘肃兰州730030 [4]兰州大学第二医院肿瘤内科,甘肃兰州730030

出　　处：《细胞与分子免疫学杂志》2020年第5期385-389,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81470808,81770525);甘肃省自然科学基金(18JR3RA326);兰州大学第二医院“萃英科技创新”计划(CY2019-QN16)。

摘　　要：目的研究白细胞介素1β(IL-1β)和肿瘤坏死因子α(TNF-α)对SD大鼠肠平滑肌细胞(ISMC)胶质细胞源性神经营养因子(GDNF)及其受体原癌基因受体酪氨酸激酶(Ret)和GDNF家族受体α1(GFRα1)表达的影响。方法改良酶消化法分离SD大鼠ISMC,免疫细胞化学染色法检测观察平滑肌细胞特异性标志物α平滑肌肌动蛋白(α-SMA)及结蛋白(desmin)的表达;单独或联合使用IL-1β和TNF-α处理ISMC 48 h,用实时定量PCR检测各组GDNF、Ret及GFRα1 mRNA相对表达水平。结果利用改良酶消化法获得了高纯度的大鼠ISMC,其α-SMA及desmin阳性率均大于95%;与空白对照组相比,TNF-α能促进ISMC表达GDNF,抑制其表达GFRα1,而对Ret的表达无明显影响,IL-1β能抑制ISMC表达Ret,对GDNF及GFRα1的表达无明显影响;IL-1β联合TNF-α能显著促进ISMC表达GDNF,抑制其表达Ret及GFRα1。结论IL-1β联合TNF-α能促进ISMC表达GDNF,抑制其表达GDNF受体Ret及GFRα1。Objective To observe the effects of interleukin 1β(IL-1β)and tumor necrosis factorα(TNF-α)on glial cell line-derived neurotrophic factor(GDNF)and its receptors in rat intestinal smooth muscle cells(ISMCs).Methods ISMCs were isolated by modified enzyme digestion in SD rats,and the positive rates of smooth muscleα-actin(α-SMA)and desmin were observed by immunocytochemical staining.ISMCs were treated with IL-1βand TNF-αfor 48 hours alone or in combination.The relative mRNA expression of GDNF,receptor tyrosine kinase(Ret)and GDNF family receptor alpha-1(GFRα1)were measured by real-time quantitative PCR.Results High-purity ISMCs were obtained by modified enzyme digestion,and over 95%of the cells expressedα-SMA and desmin.Compared with the control group,TNF-αcould promote the expression of GDNF and inhibit GFRα1,but had no effects on Ret.In contrast,IL-1βcould inhibit Ret but had no effects on GDNF and GFRα1.Notably,when IL-1βand TNF-αwere combined,the expression of GDNF was up-regulated,while RET and GFRα1 were down-regulated.Conclusion IL-1βand TNF-αcan up-regulate the expression of GDNF and down-regulate its receptors in rat ISMCs.

关 键 词：白细胞介素1β(IL-1β) 肿瘤坏死因子α(TNF-α) 肠平滑肌细胞(ISMC) 胶质细胞源性神经营养因子(GDNF) 受体酪氨酸激酶(Ret) GDNF家族受体α1(GFRα1) 

分 类 号：R516.1[医药卫生—内科学] R392.1[医药卫生—临床医学]