强直性脊柱炎患者外周血单个核细胞的磷酸化蛋白质组学分析  

Phosphoproteomic analysis of peripheral blood mononuclear cells of ankylosing spondylitis patients

作　　者：罗凤婷 姜娜 王浩 邵先锋 陈瑞冰 白人骁[5] 王毅[2] LUO Fengting;JIANG Na;WANG Hao;SHAO Xianfeng;CHEN Ruibing;BAI Renxiao;WANG Yi(Department of Genetics,School of Basic Medicine,Tianjin Medical University,Tianjin 300070;Department of Clinical Laboratory,Tianjin Hospital,Tianjin 300142;Eye Hospital,Tianjin Medical University,Tianjin 300020;School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072;Department of Rheumatology and Immunology,Tianjin Hospital,Tianjin 300142,China)

机构地区：[1]天津医科大学基础医学院遗传学系,天津300070 [2]天津医院检验科,天津300142 [3]天津医科大学眼科医院,天津300020 [4]天津大学药物科学与技术学院,天津300072 [5]天津医院风湿免疫科,天津300142

出　　处：《细胞与分子免疫学杂志》2020年第5期444-450,共7页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(21575103)。

摘　　要：目的探讨强直性脊柱炎(AS)患者外周血单个核细胞(PBMC)中蛋白磷酸化修饰的变化及其在AS中的潜在调控作用。方法分别提取20例未用药AS患者、15例用药AS患者和20例健康人群的PBMC,进行蛋白提取、胰蛋白酶消化、磷酸化肽段富集和质谱检测,并通过生物信息学分析筛选与AS相关的磷酸化蛋白,同时分析这些蛋白在治疗后表达水平的变化。结果共检测到显著差异磷酸化肽段1561个,上调756个,来自472个蛋白,下调805个,来自363个蛋白。基因注释富集(GO)分析表明,差异磷酸化蛋白主要参与中性粒细胞激活、血小板聚集等生物过程。京都基因与基因组百科全书(KEGG)通路富集分析表明,感染、血小板激活、T细胞受体、B细胞受体信号通路等可能与AS密切相关。结论发现并系统性描绘了AS患者外周血PBMC内蛋白磷酸化的改变,对了解AS的发病和疾病进展机制具有重要意义。Objective To investigate the changes of protein phosphorylation in peripheral blood mononuclear cells(PBMCs)of patients with ankylosing spondylitis(AS)and its potential regulatory role in AS.Methods PBMCs were obtained from 20 cases of AS without treatment,15 cases of AS with drug treatment,and 20 matched healthy controls.Protein extraction,trypsin digestion,phosphorylated peptide enrichment and mass spectrometric analyses were performed.AS-related phosphorylated proteins were screened by bioinformatics analysis,and the changes of expression levels of these proteins after treatment were analyzed.Results A total of 1561 significantly differential phosphorylated peptides were detected,of which 756 peptides from 472 proteins were up-regulated,and 805 from 363 proteins were down-regulated.GO enrichment analysis showed that the proteins with altered phosphorylation were mainly involved in biological processes such as neutrophil activation and platelet aggregation.The enrichment analysis of KEGG pathway showed infection,platelet activation,T cell receptors and B cell receptor signaling pathways might be closely related to AS.Conclusion This study systematically depicted the change of protein phosphorylation in PBMCs of AS patients,providing important information to understand the pathogenesis and disease progression mechanism of AS.

关 键 词：强直性脊柱炎 磷酸化蛋白质组学 蛋白磷酸化 信号通路 生物信息学 

分 类 号：R857.3[医药卫生—航空、航天与航海医学] R34[医药卫生—临床医学]