Farnesol纳米胶束的构建及对变形链球菌的抑菌作用研究  

Construction of Water-soluble Farnesol Nano-micelle and Its Antibacterial Effect on Streptococcus Mutans

作　　者：赵霞[1,2] 徐嘉昊 夏商 张晨 王万春 ZHAO Xia;XU Jiahao;XIA Shang;ZHANG Chen;WANG Wanchun(School of Stomatology, Qingdao University, Qingdao 266003, China;Department of Pediatric Stomatology, Qingdao Stomatological Hospital, Qingdao 266001, China;School of Pharmacy, Ocean University of China, Qingdao 266100, China;Department of Oral Mucosa, Qingdao Stomatological Hospital, Qingdao 266001, China)

机构地区：[1]青岛大学口腔医学院,山东青岛266003 [2]青岛市口腔医院儿童口腔科,山东青岛266001 [3]中国海洋大学药学院,山东青岛266100 [4]青岛市口腔医院口腔黏膜科,山东青岛266001

出　　处：《口腔医学研究》2020年第9期875-878,共4页Journal of Oral Science Research

基　　金：青岛市2019年度医药科研指导计划(编号:2019-WJZD138);青岛市医疗卫生重点学科建设项目。

摘　　要：目的:构建水溶性Farnesol纳米胶束,并研究其对变形链球菌的抑菌作用。方法:采用乳化溶剂扩散法制备Farnesol纳米胶束。通过Malvern粒度仪检测粒径、聚合物分散性系数(PDI)、Zeta电位3项指标,计算载药量及包封率;并考察其稳定性、水溶性、体外释放行为等。通过扫描电镜评价Farnesol纳米胶束对变形链球菌菌斑生物膜形态的影响。采用微板法检测菌悬液中钙离子浓度,探讨Farnesol纳米胶束对变形链球菌产酸及釉质脱矿的抑制作用。结果:Farnesol纳米胶束水溶液澄清,无沉淀。粒径为(19.78±0.21)nm,Zeta电势为(-12.2±0.4)mV,PDI为0.089±0.040,载药量为(8.3±0.5)%,包封率为(46.89±1.10)%,12 h累计释放70%。该纳米胶束能够抑制变形链球菌抑制菌斑生物膜的形成,降低产酸能力抑制釉质脱矿,且呈浓度依赖性。结论:Farnesol纳米胶束制备良好,具有水溶性、稳定性和缓释性等特点,并可抑制变形链球菌的生长,降低其产酸能力,对龋病的防治有重要的临床指导价值。Objective:To construct water-soluble Farnesol nano-micelle and study its antibacterial effect on streptococcus mutans.Methods:Farnesol micelles were prepared by emulsion solvent diffusion method.Particle size,polymer dispersion coefficient(PDI),and zeta potential were measured by Marvin laser particle size analyzer.It’s stability,water solubility,and in vitro release behavior were investigated.The effect of Farnesol nano-micelles on the morphology of streptococcus mutans plaque biofilm was evaluated by SEM.The calcium ion concentration in the bacterial suspension was detected by microplate method,and the inhibitory effect of Farnesol nano-micelles on acid production and enamel demineralization of streptococcus mutans was investigated.Results:Farnesol nano-micelle aqueous solution was clarified without precipitation.The particle size was(19.78±0.21)nm,the zeta potential was(-12.2±0.4)mV,the PDI was(0.089±0.040),the drug loading was(8.3±0.5)%,the encapsulation rate was(46.89±1.10)%,and the cumulative release was 70%at 12h.The nanometer micelle could inhibit the formation of plaque biofilm by streptococcus mutans,reduce the acid-producing capacity,and inhibit the demineralization of enamel in a concentration-dependent way.Conclusion:Farnesol micelles are well prepared and have the characteristics of water solubility,stability,and slow release.It can inhibit the growth of streptococcus mutans and reduce its acid producing capacity,which has important clinical value for the prevention and treatment of caries.

关 键 词：法尼醇 纳米胶束 变形链球菌 菌斑生物膜 细菌产酸 

分 类 号：R37[医药卫生—病原生物学]