Transcriptome exploration to provide a resource for the study of Auricularia heimuer  

作　　者：Jian Zhang Tingting Sun Shixin Wang Li Zou 

机构地区：[1]College of Forestry,Northeast Forestry University,Harbin 150040,People’s Republic of China [2]Department of Food Engineering,Harbin University,Harbin 150086,People’s Republic of China

出　　处：《Journal of Forestry Research》2020年第5期1881-1887,共7页林业研究（英文版）

基　　金：The work was supported by the Fundamental Research Funds for the Central Universities,(Project No.2572017CF01);the Harbin University Scientific Research Foundation project,(Project No.HUDF2018105).

摘　　要：Auricularia heimuer,an edible jelly fungus,is in considerable demand in Asia due to its high nutritive,economic and medicinal values.RNA-Seq was used to investigate and analyze the mycelium transcriptome of A.heimuer for gene discovery.A total of 26,857 unigenes with an N50 length of 1333 bp were assembled by de novo sequencing.In addition,unigenes were annotated by publicly available databases,including gene descriptions,gene ontology(GO),clusters of orthologous group(COG),Kyoto Encyclopedia of Genes and Genomes(KEGG)metabolic pathways,and protein family(Pfam)terms.A.heimuer was also studied for its wood degradation ability.Thirty-eight putative FOLymes(fungal oxidative lignin enzymes)and 251 CAZymes(carbohydrate-active enzymes)were located from A.heimuer transcriptome.Our study provides a comprehensive sequence resource for A.heimuer at the transcriptional level,which will lay a strong foundation for functional genomics studies and gene discovery of this promising fungus.

关 键 词：Auricularia heimuer TRANSCRIPTOME Carbohydrate active enzymes Lignin degradation Nextgeneration sequencing 

分 类 号：S567.3[农业科学—中草药栽培]