PPARγ激动剂对COPD大鼠炎症及血管重塑指标的影响  

Effects of PPARγagonists on inflammation and vascular remodeling in COPD rats

作　　者：王琛[1] 李智[1] 谭林 WANG Chen;LI Zhi;TAN Lin(Intensive Care Unit, Qingdao Municipal Hospital, Qingdao University, Qingdao 266071, Shandong, China)

机构地区：[1]青岛大学附属青岛市市立医院重症医学科,青岛266071

出　　处：《中国临床医学》2020年第4期670-673,共4页Chinese Journal of Clinical Medicine

基　　金：山东省医药卫生科技发展计划项目(2009-H2028)。

摘　　要：目的:探讨PPARγ激动剂罗格列酮与慢性阻塞性肺疾病(COPD)大鼠的血管重塑、炎症指标白介素-8(IL-8)的相关性。方法:36只Wistar大鼠随机分为非药物治疗组(A组)、药物治疗组(B组)、空白对照组(C组),A、B组用烟熏、低氧、脂多糖(LPS)的方法建立COPD大鼠模型;B组第3周起同时给予盐酸罗格列酮药物治疗,C组给予相同体积的生理盐水模拟造模和治疗。结果:血管壁病理示B组血管壁重塑、胶原蛋白的沉积及血管周围中性粒细胞浸润情况较A组减轻,但仍较C组严重。A组、B组、C组之间肺血管壁/肺血管总面积(MA%)差异具有统计学意义(P<0.01),A组MA%较C组明显升高,B组MA%较A组明显下降。炎性指标IL-8在3组之间差异具有统计学意义(P<0.01),其中A组明显高于B组,B组高于C组。3组的肺组织中PPARγ转录水平差异具有统计学意义(P<0.01),C组、B组、A组肺组织中PPARγ转录水平逐渐下降,其中A组明显高于B组,B组高于C组。Pearson分析显示B组大鼠中PPARγ转录水平与MA%比值、炎症指标IL-8均负相关(r=-0.662、-0.615;P<0.05),而B组中MA%与IL-8正相关(r=0.980,P<0.05)。结论:PPARγ激动剂罗格列酮通过上调PPARγ转录水平抑制COPD大鼠肺部IL-8的升高和血管周围中性粒细胞聚集、抑制了肺血管壁的重塑。Objective:To explore the effects of rosiglitazone hydrochloride on vascular remodeling and IL-8 in rat models of chronic obstructive pulmonary disease(COPD).Methods:Thirty-six Wistar rats were randomized to three groups:non-treatment group(group A),treatment group(group B),and blank group(group C).Rat model of COPD was established in groups A and B by using smoking,hypoxia and intratracheal lipopolysaccharide.The rats in group B were treated with rosiglitazone from week 3,and those in group C were given identical dose of normal saline.Results:The vascular wall pathology showed that the vascular wall remodeling,collagen deposition,and perivascular neutrophils infiltration in group B were alleviated than in group A,but more serious than in group C.The ratio of pulmonary vascular wall/total vascular area in group A were higher than that in groups C and B(P<0.01).The level of IL-8 in group A were higher than in groups C and B(P<0.01).PPARγmRNA in lung tissue increased in order of groups A,B,and C(P<0.01).Pearson analysis showed that in group B,the levels of PPARγmRNA were negatively correlated with the ratio of pulmonary vascular wall/total vascular area and the level of IL-8(r=-0.662 and-0.615,P<0.05).Conclusions:Rosigltazone inhibits the remodeling of the pulmonary vascular wall in COPD rats by upregulating transcription of PPARγand inhibiting the increase of IL-8 and the infiltration of neutrophils around the vascular wall.

关 键 词：肺性 慢性阻塞性 血管重塑 炎症 PPARΓ IL-8 

分 类 号：R56.3[医药卫生—呼吸系统] R543.2[医药卫生—内科学]