低温离子体与红外光谱技术及其在灵芝诱变育种中的应用研究进展  

A review:low-temperature plasma technology combined with infrared spectroscopy and its application in mutation breeding of Ganoderma lucidum

作　　者：黄青[1,2] 马玉涵 张倩倩[1,2] 姚国华 何华奇 韦达 HUANG Qing;MA Yuhan;ZHANG Qianqian;YAO Guohua;HE Huaqi;WEI Da(Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China;Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei 230031, China;College of Life and Health Sciences, Anhui Science and Technology University, Fengyang 233100, China)

机构地区：[1]中国科学院合肥物质科学研究院,强磁场与离子束物理生物学重点实验室,安徽合肥230031 [2]中国科学院智能机械研究所,安徽合肥230031 [3]安徽科技学院生命与健康科学学院,安徽凤阳233100

出　　处：《安徽大学学报（自然科学版）》2020年第5期1-15,共15页Journal of Anhui University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(11635013,11775272,11475217)。

摘　　要：微生物是很多化工产品、营养成分和药用成分的重要来源,通过对微生物实施诱变育种,可获得高产有用成分的菌株.低温等离子体诱变技术具有使用方便、有效突变率高等优点,与红外光谱技术相结合,可对有用和关键产物进行无损、快速定性和定量分析,有利于从大量的突变菌株中快速筛选出所需的优良菌株.该文概述低温等离子体和红外光谱技术在微生物育种中的应用,介绍低温等离子体和红外光谱(包括中红外和近红外光谱)技术在灵芝诱变育种和品质检测方面的最新研究进展,展望其未来的应用前景.Microorganisms are important sources of many chemical products,nutrients and medicinal ingredients.Through mutation breeding technology,microbial strains with high yields and useful components can be acquired.Low-temperature plasma(LTP)mutagenesis technology has many advantages,such as easy use and high mutation rate.Combined with infrared spectroscopy,the key products can be analyzed quickly,non-destructively,qualitatively and quantitatively,which is conducive to the rapid screening of a large number of mutant strains.In this paper,the application of low temperature plasma mutagenesis and infrared spectroscopy screening technology in the microbial mutation breeding was reviewed.The latest research progress of low-temperature plasma and infrared spectroscopy(including mid-infrared and near-infrared spectroscopy)technology in the mutation breeding and quality inspection of Ganoderma lucidum was introduced,and its future application prospects were expected.

关 键 词：低温等离子体 红外光谱 微生物 诱变 灵芝 近红外光谱 高通量筛选 

分 类 号：Q632[生物学—生物物理学] O433[机械工程—光学工程]