柚皮苷对缺氧/复氧损伤心肌细胞Caspase-3活性及IRE1α表达的影响  

Effect of naringin on Caspase-3 activity and IRE1αexpression in myocardial cells injured by hypoxia/reoxygenation

作　　者：刘丹[1,2] 熊书 马羚[1,2] 马丽华 张永慧[1,2] LIU Dan;XIONG Shu;MA Ling;MA Li-hua;ZHANG Yong-hui(Chongqing Three Gorges Medical College,Chongqing 404120,China)

机构地区：[1]重庆三峡医药高等专科学校,重庆404120 [2]重庆市抗肿瘤天然药物工程技术研究中心,重庆404120

出　　处：《中国生物制品学杂志》2020年第8期904-907,共4页Chinese Journal of Biologicals

基　　金：重庆市卫生和计划生育委员会中医药科技项目(ZY201703-077);重庆三峡医药高等专科学校重点项目(2016xzz02)。

摘　　要：目的研究柚皮苷(naringin,Nar)对缺氧/复氧(hypoxia/reoxygenation,H/R)损伤心肌细胞中Caspase-3活性及IRE1α蛋白表达水平的影响。方法建立H9c2心肌细胞H/R损伤模型,将H9c2心肌细胞随机分为5组:正常对照组(C组)、缺氧/复氧组(H/R组)、H/R+Nar低、中、高剂量组。C组心肌细胞正常培养至实验结束;H/R组行缺氧4 h后复氧24 h;H/R+Nar低、中、高剂量组分别于缺氧前6 h给予10、20、40μg/mL Nar培养,再行缺氧4 h后复氧24 h。采用酶标仪法检测各组心肌细胞中Caspase-3活性,Western blot法检测各组心肌细胞中IRE1α蛋白的表达水平。结果与C组比较,H/R组心肌细胞中Caspase-3活性及IRE1α表达水平明显升高(P均<0.05);与H/R组及H/R+Nar 10μg/mL剂量组比较,H/R+Nar 20、40μg/mL剂量组心肌细胞中Caspase-3活性及IRE1α蛋白表达水平均明显降低(P均<0.05)。结论Nar预处理可降低Caspase-3活性,下调IRE1α蛋白表达水平。Objective To investigate the effect of naringin(Nar)on Caspase-3 activity and IRE1αexpression level in myocardial cells injured by hypoxia/reoxygenation(H/R).Methods H9 c2 cell model of H/R injury was established.The cells were randomly divided into five groups:normal control(C),hypoxia/reoxygenation(H/R)as well as H/R+low,moderate and high dose Nar groups.The cells in C group were normally cultured until the end of the experiment,while those in H/R group were treated by hypoxia for 4 h followed by reoxygenation for 24 h.However,the cells in H/R+low,moderate and high dose Nar groups were cultured with 10,20 and 40μg/mL Nar 6 h before hypoxia respectively,followed by reoxygenation for 24 h.The activities of Caspase-3 in various groups were determined by colorimetric method,while the expression levels of IRE1αby Western blot.Results Compared with those in C group,both the activity of Caspase-3 and the expression level of IRE1αin H/R group increased significantly(each P<0.05).However,compared with those in H/R and H/R+low dose Nar group,the Caspase-3 activities and IRE1αexpression levels in H/R+moderate dose Nar and H/R+high dose Nar groups decreased significantly(each P<0.05).Conclusion The pretreatment with Nar may decrease the activity of Caspase-3 and down-regulate the expression level of IRE1α.

关 键 词：柚皮苷 缺氧/复氧 心肌细胞 Caspase-3 IRE1α 

分 类 号：R966[医药卫生—药理学]