甘肃省人感染和外环境来源的H9N2禽流感病毒基因特征分析  

Analysis on genetic characteristics of H9N2 avian influenza virus isolated from human infection and external environment in Gansu province

作　　者：李保娣[1] 何军[2] 李红育[1] 张慧[1] 徐丛杉 于德山[1] 李建兵[3] 何健[1] Li Baodi;He Jun;Li Hongyu;Zhang Hui;Xu Congshan;Yu Deshan;Li Jianbing;He Jian(Gansu Provincial Center for Disease Control and Prevention,Lanzhou 730020,China;Anhui Provincial Center for Disease Control and Prevention,Hefei 230601,China;Jiayuguan Center for Disease Control and Prevention,Jiayuguan 735100,China)

机构地区：[1]甘肃省疾病预防控制中心,兰州730020 [2]安徽省疾病预防控制中心,合肥230601 [3]嘉峪关市疾病预防控制中心,735100

出　　处：《中华流行病学杂志》2020年第8期1345-1351,共7页Chinese Journal of Epidemiology

基　　金：国家科技重大专项(2017ZX10103006001,2017ZX10104001);甘肃省卫生行业科研计划(GSWSKY2017-28);安徽省卫计委2017年科研计划(2017jk005)。

摘　　要：目的分析甘肃省发现的人感染H9N2禽流感病毒分离株全基因组特征。方法对甘肃省2016年流感样病例中发现并确诊的1例人感染H9N2禽流感病毒病例进行病原学分析,并使用MEGA 7.0等软件解析该毒株全基因组特征。结果该毒株HA、NA、MP、NP、NS、PA、PB1和PB2各个基因片段与甘肃省2014-2019年外环境中分离获得的H9N2禽流感病毒各基因片段高度相似,且均>90%;其HA基因属于BJ/94-like支系,PB2和MP属于G1/97-like支系,PB1、PA、NS和NP基因属于F/98-like支系,MP和PB2与H7N9、H10N8和H5N6亲缘关系较近;氨基酸序列比对发现HA裂解位点呈PSRSSR↓GLF排列,发生H183N和Q226L突变,有7个HA糖基化位点;NA茎部62~64位均缺失ITE 3个氨基酸;M2-31N、NS1-42S、PA-356R和PA-409N突变。结论人感染H9N2禽流感病毒为偶发感染,但甘肃省外环境中分离的H9N2禽流感病毒具有一系列哺乳动物适应性分子标记,提示人群感染风险较高,需多部门加强监测,共同应对流感大流行。Objective To analyze the genomic characteristics of human infection with H9N2 avian influenza virus in Gansu province.Methods The etiological analysis was conducted for human infection with H9N2 avian influenza virus detected in influenza like illness cases in northwestern China in 2016.Molecular bioinformatics Mega 7.0 software was used to analyze the full genomic sequences of the viral isolate.Results The gene fragments of HA,NA,MP,NP,NS,PA,PB1 and PB2 of the isolate were highly similar(>90%)to those of H9N2 avian influenza virus strain isolated in external environment in Gansu from 2014 to 2019.The HA gene belonged to BJ/94-like branch,PB2 and MP belonged to G1/97-like branch,and the PB1,PA,NS,and NP genes belonged to F/98-like branch.MP and PB2 were closely related to H7N9,H10N8 and H5N6 viruses.Amino acid sequence alignment showed that the HA cleavage site was arranged in PSRSSR↓GLF,H183N and Q226L mutated which included 7 HA glycosylated sites;62-64 sites of NA absented 3 amino acids(ITE);and M2-31N,NS1-42S,PA-356R,and PA-409N mutated.Conclusions Apparently,this case of human infection with human infection with H9N2 avian influenza virus was an incidental.However,the isolates of H9N2 influenza virus in external environment of Gansu had a series of mammalian adaptive molecular markers,suggesting that the risk of human infection is higher.It is necessary to strengthen the surveillance by multi departments to deal with influenza pandemic.

关 键 词：H9N2禽流感病毒 全基因组 序列比对 人感染 

分 类 号：R511.7[医药卫生—内科学]