纳米银杂化的载辛伐他汀PLGA微球合成及性质研究  

Synthesis and identification of nanosilver-hybridized PLGA microsphere loaded with simvastatin

作　　者：张金明[1] 刘宝会 欧阳昭广 贾智[1] 刘大勇[1] Zhang Jinming;Liu Baohui;Ouyang Zhaoguang;Jia Zhi;Liu Dayong(Tianjin Medical University,Stomatological Hospital of Tianjin Medical University,Tianjin 300070,China)

机构地区：[1]天津医科大学口腔医院,300070

出　　处：《国际生物医学工程杂志》2020年第3期199-206,共8页International Journal of Biomedical Engineering

基　　金：国家自然科学基金(81670953)。

摘　　要：目的制备纳米银(AgNPs)杂化的载辛伐他汀(SIM)聚乳酸-羟基乙酸共聚物(PLGA)微球,评价其体外缓释效果。方法采用乳化-溶剂挥发法制备载SIM的PLGA微球。使用丝素蛋白(SF)并通过其疏水作用对载SIM的PLGA微球表面进行改性;通过静电吸附用壳聚糖(CTS)及AgNPs进一步对微球表面进行改性,制备AgNPs吸附CTS修饰SF包覆的载SIM的PLGA微球。使用扫描电子显微镜、傅里叶变换红外光谱仪、能谱仪、Zeta电位仪对载药微球进行分析,并考察其体外释放性能。结果所制备的PLGA微球的平均直径约为9.67μm。傅里叶变换红外光谱和能谱结果表明,成功构建了AgNPs杂化后的载SIM的微球;Zeta电位结果表明载药微球处于稳定的状态;体外释放结果表明,载药微球有较好的体外释放效果,能延缓药物释放速率并延长药物释放时间。结论SF修饰的AgNPs杂化的载SIM的PLGA微球具有抑菌与成骨作用,且表现出较好的体外释放效果,可应用于口腔内的局部缓释给药,在牙周炎治疗方面具有潜在的应用价值。Objective To prepare nanosilver-hybridized polylactic acid-glycolic acid copolymer(PLGA)microspheres loaded with simvastatin(SIM),and to evaluate its sustained release effect in vitro.Methods The emulsification-solvent evaporation method was used to prepare SIM-loaded PLGA microspheres.Silk fibroin(SF)was used to modify the surface of SIM-loaded PLGA microspheres by hydrophobic interaction.Then,the microspheres were continually modified by electrostatic adsorption to chitosan(CTS)and nano-silver(AgNPs)to prepare SF-AgNPs-CTS-SF-SIM-PLGA microspheres.Scanning electron microscope,Fourier transform infrared spectrometer,energy spectrometer,Zeta potential meter were used to analyze the SIM-loaded microspheres.The external release properties of the SIM-loaded microspheres were also investigated.Results The average diameter of the prepared PLGA microspheres was about 9.67μm.The results of Fourier transform infrared spectroscopy and energy spectroscopy showed that the AgNPs-CTS-SF-SIM-PLGA microspheres have been successfully constructed.The Zeta potential results indicated that the SIM-loaded microspheres were all in a stable state.The in vitro release results showed that the SF-AgNPs-CTS-SF-SIM-PLGA microspheres had a good in vitro release effect,could delay the drug release rate and prolong the drug release time.Conclusions The SF-AgNPs-CTS-SF-SIM-PLGA microspheres have antibacterial and osteogenic effects,and exhibit a good in vitro release effect.They can be used for local sustained-release administration in the oral cavity,which make makes them potentially useful in the treatment of periodontitis.

关 键 词：辛伐他汀 聚乳酸-羟基乙酸共聚物 纳米银 微球 药物释放体系 

分 类 号：R943[医药卫生—药剂学]