石菖蒲有效成分对APP/PS1双转基因小鼠GAP-43表达的影响  

Effects of Active Ingredients of Acorus Tatarindii on GAP-43 Expression in APP/PS1 Double Transgenic Mice

作　　者：孙晓雪[1] 王宁宁 李琳 荣华 SUN Xiaoxue;WANG Ningning;LI Lin;RONG Hua(The Second Affiliated Hospital of Qiqihar Medical University,Qiqihar 161006,China;不详)

机构地区：[1]齐齐哈尔医学院附属第二医院,黑龙江齐齐哈尔161006 [2]齐齐哈尔医学院医药科学研究院,黑龙江齐齐哈尔161006

出　　处：《中国医学创新》2020年第25期21-25,共5页Medical Innovation of China

基　　金：齐齐哈尔市科学技术局项目(SFGG-201755)。

摘　　要：目的:观察石菖蒲有效成分β-细辛醚对APP/PS1双转基因小鼠生长相关蛋白43(growthassociated protein 43,GAP-43)表达的影响,探讨β-细辛醚对APP/PS1双转基因小鼠神经突触可塑性的保护作用及对阿尔茨海默病(AD)的治疗作用机制。方法:选取2019年3-4月40只2月龄APP/PS1双转基因小鼠随机分为模型组,盐酸多奈哌齐组,低剂量组及高剂量组,每组10只,选用10只相同月龄C57BL/6小鼠为空白对照组。空白对照组与模型组分别给予0.1 mL/10 g 0.9%氯化钠溶液,盐酸多奈哌齐组给予0.33 mg/(kg·d)盐酸多奈哌齐,低剂量组给予β-细辛醚25 mg/(kg·d),高剂量组给予50 mg/(kg·d)β-细辛醚,五组均连续灌胃4周。采用Morris水迷宫法检测并比较各组学习记忆能力,采用免疫荧光法检测并比较各组海马区GAP-43蛋白的表达变化,采用RT-PCR技术检测并比较各组海马区GAP-43mRNA表达水平。结果:模型组、盐酸多奈哌齐组、低剂量组与高剂量组逃避潜伏期均高于空白对照组,且跨越平台次数均低于空白对照组(P<0.05)。盐酸多奈哌齐组、低剂量组与高剂量组逃避潜伏期均低于模型组,且跨越平台次数高于模型组(P<0.05)。盐酸多奈哌齐组与高剂量组逃避潜伏期均低于低剂量组(P<0.05)。盐酸多奈哌齐组与高剂量组跨越平台次数均高于低剂量组,且高剂量组高于盐酸多奈哌齐组(P<0.05)。模型组、盐酸多吗哌齐组、低剂量组与高剂量组GAP-43蛋白与GAP-43mRNA表达量均高于空白对照组(P<0.05)。盐酸多奈哌齐组、低剂量组与高剂量组GAP-43蛋白与GAP-43mRNA表达量均低于模型组(P<0.05)。结论:β-细辛醚具有改善AD的作用,并且作用机制与调控小鼠海马神经突触可塑性相关因子GAP-43的表达相关。Objective:To observe the effect ofβ-asarone on the expression of growth-associated protein 43(GAP-43)in APP/PS1 double transgenic mice,and to explore the protective effect ofβ-asarone on the synaptic plasticity of APP/PS1 double transgenic mice and the therapeutic mechanism ofβ-asarone on Alzheimer’s disease(AD).Method:From March to April 2019,a total of 40 APP/PS1 double transgenic mice with 2 month old were randomly divided into model group,Donepezil Hydrochloride group,low-dose group and high-dose group,with 10 mice in each group.10 mice with C57BL/6 in the same month age were selected as blank control group.The blank control group and model group were given 0.1 mL/10 g 0.9%sodium chloride solution.The Donepezil Hydrochloride group was given 0.33 mg/(kg·d)Donepezil Hydrochloride.The Low-dose group was given 25 mg/(kg·d)β-asarone.The high-dose group was given 50 mg/(kg·d)β-asarone.All the five groups were given gavage for 4 weeks.The learning and memory ability of each group were detected and compared by Morris water maze method.The expression of GAP-43 protein of each group were detected and compared by immunofluorescence method.The expression of GAP-43 mRNA in hippocampus of each group were detected and compared by RT-PCR.Result:The escape latency of model group,the Donepezil Hydrochloride group,low-dose group and high-dose group were higher than that of blank control group,and the number of crossing the platform were lower than that of the blank control group(P<0.05).The escape latency of the Donepezil Hydrochloride group,low-dose group and high-dose group were lower than that of model group,and the number of crossing the platform were higher than that of model group(P<0.05).The escape latency of the Donepezil Hydrochloride group and the high-dose group were lower than that of low-dose group(P<0.05).The number of crossing the platform in the Donepezil Hydrochloride group and the high dose group were higher than those in the low-dose group,and the high dose group was higher than the Donepezil Hydroc

关 键 词：阿尔茨海默病 Β-细辛醚 APP/PS1双转基因小鼠 神经生长相关蛋白43 

分 类 号：R285.5[医药卫生—中药学]