荧光假单胞杆菌磷脂酶B界面催化分子机制初探  

Interfacial catalytic mechanism of Pseudomonas fluorescens phospholipase B

作　　者：赵依威 姜芳燕 李春 戴大章 ZHAO Yiwei;JIANG Fangyan;LI Chun;DAI Dazhang(School of Chemistry and Chemical Engineering,Beijing Institute of Technology,Beijing 100081,China)

机构地区：[1]北京理工大学化学与化工学院,北京100081

出　　处：《化工学报》2020年第9期4255-4259,共5页CIESC Journal

基　　金：国家自然科学基金项目(21276025)。

摘　　要：基于荧光假单胞杆菌磷脂酶B(Pf-PLB)一级序列,分析了Pf-PLB的相关理化性质及其二级结构,并采用同源建模的方法对Pf-PLB的三级结构进行了预测;基于其预测结构,提出“掀盖进入,侧向离去”假说模型,初步探讨了Pf-PLB界面催化的可能机制;采用定点突变的方法对Pf-PLB的结构进行改造,结合突变体Pf-PLB的催化特性对所提出的机制模型进行了验证。The physicochemical property and secondary structure of Pseudomonas fluorescens phospholipase B(Pf-PLB)were analyzed base on its primary structure,and the tertiary structure of Pf-PLB was predicated.A“lid-in,side-out”hypothesis model was put forward to illuminate the possible interfacial catalytic mechanism of Pf-PLB.The structure of Pf-PLB was modified by site-directed mutagenesis,and the proposed mechanism model was verified by combining with the catalytic properties of mutant Pf-PLB.

关 键 词：磷脂酶B 结构预测 催化机制 

分 类 号：TQ028.8[化学工程]