原花青素联合左氧氟沙星对尿路致病性大肠杆菌侵袭小鼠膀胱的影响  

Effect of Proanthocyanidins and Levofloxacin on Urinary Tract Pathogenic Escherichia Coli Invading Bladder of Mice

作　　者：沙凯辉[1] 刘同刚[2] 张晓丽[1] SHA Kaihui;LIU Tonggang;ZHANG Xiaoli(School of Nursing,Binzhou Medical University,Binzhou 256600,China;不详)

机构地区：[1]滨州医学院护理学院,山东滨州256600 [2]滨州医学院附属医院

出　　处：《中外医学研究》2020年第24期177-178,共2页CHINESE AND FOREIGN MEDICAL RESEARCH

基　　金：山东省医药卫生科技发展计划项目(2017WS886)。

摘　　要：目的:探讨原花青素联合左氧氟沙星对尿路致病性大肠杆菌(UPEC)侵袭小鼠膀胱的抑制作用。方法:将雌性C57BL/6小鼠60只随机分为四组,分别为空白对照组、左氧氟沙星组、原花青素组、原花青素+左氧氟沙星组,每组15只。将UPEC J96稀释菌液注入各组小鼠膀胱内,感染2 h后在无菌条件下摘取膀胱,制备成组织匀浆,稀释后涂布于LB琼脂平板上,以37 ℃孵育过夜。采用平板菌落计数法检测各组UPEC J96相对侵袭率。结果:左氧氟沙星组、原花青素组、原花青素+左氧氟沙星组UPEC J96相对侵袭率低于空白对照组,原花青素+左氧氟沙星组UPEC J96相对侵袭率低于左氧氟沙星组、原花青素组,差异均有统计学意义(P<0.05);原花青素组与左氧氟沙星组UPEC J96相对侵袭率比较,差异无统计学意义(P>0.05)。结论:原花青素与左氧氟沙星联合应用更有利于抑制UPEC对小鼠膀胱的侵袭。Objective:To investigate the inhibitory effect of Proanthocyanidins and Levofloxacin on urinary tract pathogenic escherichia coli(UPEC)invading bladder of mice.Method:A total of 60 female C57BL/6 mice were randomly divided into four groups,including the blank control group,the Levofloxacin group,the Proanthocyanidins group,the Proanthocyanidins+Levofloxacin group,15 mice in each group.UPEC J96 diluent was injected into the bladder of mice in each group,and the bladder was extracted under sterile conditions 2 h after infection to prepare tissue homogenate,which was diluted and coated on LB AGAR plate and incubated overnight at 37℃.The relative invasion rate of UPEC J96 was measured by plate colony counting method.Result:The relative invasion rate of UPEC J96 in the Levofloxacin group,the Proanthocyanidins group,the Proanthocyanidins+Levofloxacin group was significantly lower than that of the blank control group,and the relative invasion rate of UPEC J96 in the Proanthocyanidins+Levofloxacin group was significantly lower than that of the Levofloxacin group and the Proanthocyanidins group,and the differences were statistically significant(P<0.05).The relative invasion rate of UPEC J96 between the Proanthocyanidins group and the Levofloxacin group was compared,and the difference was not statistically significant(P>0.05).Conclusion:The combination of Proanthocyanidins and Levofloxacin is more beneficial to the inhibition of UPEC invasion on the bladder of mice.

关 键 词：原花青素 左氧氟沙星 UPEC 侵袭 小鼠 膀胱 感染 

分 类 号：R691.3[医药卫生—泌尿科学]