丹参酮ⅡA对高脂血症大鼠PPAR信号通路蛋白表达的影响及其肝脏保护机制的研究  

Study on Effect of Tanshinone II A on Protein Expression of PPAR Signaling Pathway in Hyperlipidemia Rats and Its Protective Mechanism on Liver

作　　者：陈周明[1] 李炳荣[1] 毛卫波[1] CHEN Zhouming;LI Bingrong;MAO Weibo

机构地区：[1]丽水市中心医院,浙江丽水323000

出　　处：《新中医》2020年第16期8-11,共4页New Chinese Medicine

基　　金：2017年浙江省医药卫生科技计划项目(2017KY724)。

摘　　要：目的:探讨丹参酮ⅡA对高脂血症大鼠过氧化物酶体增殖剂激活受体(PPAR)信号通路蛋白表达的影响及其对肝脏保护的作用机制。方法:选取SPF级雄性SD大鼠27只,按照随机数字表法将大鼠分为丹参酮组、模型组和空白组,每组9只。空白组大鼠给予普通饲料,丹参酮组和模型组大鼠均给予高脂饲料,连续喂养4周。模型制备成功后,丹参酮组按10 mg/(kg·d)的剂量腹腔注射丹参酮ⅡA磺酸钠,模型组和空白组均腹腔注射等量磷酸盐缓冲生理盐水,连续8周。检测大鼠谷草转氨酶(AST)、谷丙转氨酶(ALT)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、甘油三酯(TG)和总胆固醇(TC)水平;观察大鼠肝脏组织HE染色和油红O染色结果;Western blot法检测载脂蛋白A-I(Apoa-I)、细胞色素P4504A1(CYP4A1)、过氧化物酶体增殖剂激活受体α(PPARα)蛋白表达情况。结果:与空白组比较,模型组大鼠HDL-C、CYP4A1、Apoa-I、PPARα水平均降低(P<0.05,P<0.01),AST、ALT、LDL-C、TG、TC水平均升高(P<0.05);下调蛋白175个,上调蛋白314个,共489个。与模型组比较,丹参酮组大鼠AST、ALT均降低(P<0.05),CYP4A1、Apoa-I、PPARα水平均升高(P<0.05,P<0.01),HDL-C、LDL-C、TG、TC水平比较,差异无统计学意义(P>0.05);下调蛋白148个,上调蛋白108个,共256个。结论:丹参酮ⅡA可促进高脂血症大鼠脂肪酸氧化及胆固醇逆向转运,改善PPAR信号通路,以保护肝脏。Objective:To study the effect of tanshinone IIA on protein expression of peroxisome proliferator-activated receptor(PPAR)signaling pathway in hyperlipidemia rats and its protective mechanism on liver.Methods:A total of 27 SD male rats of SPF grade were divided into the tanshinone group,the model group and the blank group according to the random number table method,nine rats in each group.Rats in the blank group were fed with normal diet,and rats in the tanshinone group and model group were fed with high-fat diet;all were fed for four weeks.After the model preparation,rats in the tanshinone group were intraperitoneally injected with 10 mg/(kg·d)tanshinone II A sodium sulfonate;the model group and the blank group were intraperitoneally injection with the same amount of phosphate-buffered saline;all groups lasted for eight weeks.The levels of aspartate transaminase(AST),alanine transaminase(ALT),low density lipoprotein-C(LDL-C),high density lipoprotein-C(HDL-C),triglyceride(TG)and total cholesterol(TC)were detected.The results of HE staining and oil red O staining were observed.The levels of apolipoprotein A-I(Apoa-I),cytochromeP4504 A1(CYP4 A1)and protein expression of peroxisome proliferators-activated receptorα(PPARα)were detected by Western Blot.Results:Compared with those in the blank group,the levels of HDL-C,CYP4 A1,Apoa-I and PPARαwere decreased in the model group(P<0.05,P<0.01),and the levels of AST,ALT,LDL-C,TG and TC were increased(P<0.05);175 proteins were downregulated and 314 proteins up-regulated,with a total of 489.Compared with those in the model group,the levels of AST and ALT were decreased in the tanshinone group(P<0.05),and the levels of CYP4 A1,Apoa-I and PPARαwere increased(P<0.05,P<0.01);there was no significant difference in the comparison of the levels of HDL-C,LDL-C,TG and TC(P>0.05);148 proteins were down-regulated and 108 proteins up-regulated,with a total of 256.Conclusion:Tanshinone IIA can promote the oxidation of fatty acids and the reverse transport of cholesterol in hyperlipid

关 键 词：高脂血症 丹参酮ⅡA PPAR 动物实验 大鼠 

分 类 号：R285.5[医药卫生—中药学]