都安山羊和隆林山羊mtDNA D-loop区遗传多态性研究  

Genetic Polymorphism of mtDNA D-loop Region in Du’an Goat and Longlin Goat

作　　者：李芳玉 黄光云[1] 王自豪[1] 滕少花[1] 曹艳红[1] 孙俊丽[1] 雷初朝[2] 廖玉英[1] LI Fangyu;HUANG Guangyun;WANG Zihao;TENG Shaohua;CAO Yanhong;SUN Junli;LEI Chuzhao;LIAO Yuying(Animal Husbandry Institute of Guangxi Zhuang Autonomous Region,Guangxi Key Laboratory of Livestock Genetic Improvement,Guangxi Nanning 530001,China;College of Animal Science and Technology,Northwest A&F University,Shaanxi Yangling 712100,China)

机构地区：[1]广西壮族自治区畜牧研究所,广西家畜遗传改良重点实验室,广西南宁530001 [2]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《中国畜牧杂志》2020年第8期69-72,共4页Chinese Journal of Animal Science

基　　金：广西创新驱动发展专项(桂科AA17204024)。

摘　　要：为探究广西都安山羊和隆林山羊2个地方品种的遗传多样性及母系起源,利用PCR测序及生物信息方法,对35只都安山羊和23只隆林山羊的mtDNA D-loop区进行多态性分析,共检测到83个变异位点,定义32种单倍型。都安山羊和隆林山羊的单倍型多样度分别为0.956和0.960,其核苷酸多样度均为0.018,平均核苷酸差异数分别为21.2和21.5,说明都安山羊和隆林山羊具有丰富的遗传多样性。构建的NJ发育树表明,都安山羊和隆林山羊主要分为A与B 2个支系,A支系与弯角野山羊(Capra aegagrus)聚在一起,而旋角野山羊(Capra falconeri)单独聚为一支,揭示弯角野山羊是都安山羊和隆林山羊的母系祖先。In order to explore the genetic diversity and maternal origin of Du’an goat and Longlin goat, the polymorphism of mtDNA D-loop region of 58 goats including 35 Du’an goats and 23 Longlin goats) was detected by PCR amplification, DNA sequencing and bioinformatics method. A total of 83 mutation sites and 32 haplotypes were detected in 58 goat D-loop regions. The haplotype diversity of Du’an goat and Longlin goat was(0.956±0.020) and(0.960±0.026), respectively, and their nucleotide diversity was the same with(0.018±0.005). The average nucleotide differences were 21.2 and 21.5, respectively. The results showed abundant genetic diversity in Du’an goat and Longlin goat. The NJ tree indicated that there were two lineages A and B in Du’an goat and Longlin goat, lineage A was clustered with Capra aegagrus, and Capra falconeri was clustered alone, which indicated that Capra aegagrus was the maternal origin of Du’an goat and Longlin goat breeds.

关 键 词：山羊 mtDNA D-loop 遗传多样性 支系 母系起源 

分 类 号：S826.2[农业科学—畜牧学]