BioPRYN酶联免疫方法检测牛早孕的应用研究  

Study on the Application of the BioPRYN Enzyme-linked Immunosorbent Assay for Diagnosis of Pregnancy in Cows

作　　者：解鑫[1] 马文丽[1] 宋晓东[1] 周国学 梁春梅[1] 喻东威[1] XIE Xin;MA Wen-li;SONG Xiao-dong;ZHOU Guo-xue;LIANG Chun-mei;YU Dong-wei(Inner Mongolia MengNiu Dairy Products Co.,Ltd,Hohhot 011517)

机构地区：[1]内蒙古蒙牛乳业(集团)股份有限公司,呼和浩特011517

出　　处：《中国奶牛》2020年第7期23-25,共3页China Dairy Cattle

基　　金：2018年国家重点研发计划重点专项(2018YFC1604202)。

摘　　要：为研究BioPRYN酶联免疫方法诊断牧场奶牛早孕的应用前景,采用BioPRYN酶联免疫方法检测两个牧场共92头奶牛配种后血液中的PSPB(pregnancy-specific protein-B)含量,从而判断奶牛的怀孕情况,之后以传统触诊法进行结果确认,评估试剂盒检测结果的准确性。结果显示:BioPRYN技术检测未孕奶牛的准确率为100.0%,怀孕奶牛的准确率为92.8%。该方法简便、快速,可以在早期对奶牛受孕情况进行大通量排查,丰富牧场奶牛早孕检测手段,同时可以配合传统检测方法及时发现流产奶牛,为规模化牧场奶牛配种管理和成本控制提供技术保障。In order to study the application prospect of BioPRYN enzyme-linked immunosorbent assay(ELISA) method for early diagnosis of pregnancy of cows in diary farms, BioPRYN ELISA method was used to detect the concentration of PBSB(pregnancy-specific protein-B) in the blood of 92 cows from two dairy farms for diagnosis of pregnancy. And then the results were confirmed by traditional palpation method, so that the accuracy of the ELISA method can be assessed. The accuracy of ELISA method for non-pregnant individuals was 100.0%, while that of pregnant ones was 92.8%. Besides, the method was simple and rapid, which could conduct large-scale investigation of cows’ pregnant status at early-term after mating, and enrich the detection methods of early pregnancy in dairy cows. Combined with the traditional detection method, the ELISA method can be used to timely discover aborted dairy cows, providing technical support for the management and cost control of large-scale dairy farms.

关 键 词：奶牛早孕检测 妊娠特异性蛋白B BioPRYN酶联免疫检测方法 触诊法 

分 类 号：S823.3[农业科学—畜牧学]